LOCUS       sequence-125984-        3716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF1 [NM_007182.4] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3716)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E251 Hs.RASSF1
FEATURES             Location/Qualifiers
     source          1..3716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1207..1306)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1683..2474
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2567..3155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3056..3075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3309..3326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3485..3512)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3604..3690)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtc gggggagcct gagctcattg agctgcggga gctggcaccc gctgggcgcg
      241 ctgggaaggg ccgcacccgg ctggagcgtg ccaacgcgct gcgcatcgcg cggggcaccg
      301 cgtgcaaccc cacacggcag ctggtccctg gccgtggcca ccgcttccag cccgcggggc
      361 ccgccacgca cacgtggtgc gacctctgtg gcgacttcat ctggggcgtc gtgcgcaaag
      421 gcctgcagtg cgcgcattgc aagttcacct gccactaccg ctgccgcgcg ctcgtctgcc
      481 tggactgttg cgggccccgg gacctgggct gggaacccgc ggtggagcgg gacacgaacg
      541 tggacgagcc tgtggagtgg gagacacctg acctttctca agctgagatt gagcagaaga
      601 tcaaggagta caatgcccag atcaacagca acctcttcat gagcttgaac aaggacggtt
      661 cttacacagg cttcatcaag gttcagctga agctggtgcg ccctgtctct gtgccctcca
      721 gcaagaagcc accctccttg caggatgccc ggcggggccc aggacggggc acaagtgtca
      781 ggcgccgcac ttccttttac ctgcccaagg atgctgtcaa gcacctgcat gtgctgtcac
      841 gcacaagggc acgtgaagtc attgaggccc tgctgcgaaa gttcttggtg gtggatgacc
      901 cccgcaagtt tgcactcttt gagcgcgctg agcgtcacgg ccaagtgtac ttgcggaagc
      961 tgttggatga tgagcagccc ctgcggctgc ggctcctggc agggcccagt gacaaggccc
     1021 tgagctttgt cctgaaggaa aatgactctg gggaggtgaa ctgggacgcc ttcagcatgc
     1081 ctgaactaca taacttccta cgtatcctgc agcgggagga ggaggagcac ctccgccaga
     1141 tcctgcagaa gtactcctat tgccgccaga agatccaaga ggccctgcac gcctgccccc
     1201 ttgggtaatc aactttcttg tacaaagttg gcattatagg aaagcattgc ttatcaattt
     1261 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgcatg caagcttaga
     1321 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1381 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1441 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1501 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1561 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1621 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1681 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     1741 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     1801 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     1861 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     1921 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     1981 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2041 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2101 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2161 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2221 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2281 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2341 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2401 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2461 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     2521 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2581 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2641 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2701 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2761 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2821 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2881 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2941 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3001 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3061 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3121 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3181 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3241 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3301 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3361 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3421 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3481 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3541 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3601 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3661 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcg
//
