LOCUS       sequence-125986-        4217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF10 [NM_001080521.2] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4217)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E253 Hs.RASSF10
FEATURES             Location/Qualifiers
     source          1..4217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1708..1807)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2184..2975
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3068..3656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3557..3576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3810..3827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3986..4013)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4105..4191)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga tccttcggaa aagaagatat cggtgtggat ctgccaggaa gagaagctgg
      241 tgtccggcct ctcccgccgc accacttgct ccgacgttgt gcgagtgctt ttggaggacg
      301 gctgccggcg gcgacggaga cagcggcgga gccggcggct ggggtcggcc ggcgacccgc
      361 atggcccggg agagctgccc gaacccccga acgaggacga cgaggacgac gacgaggcgc
      421 tgccgcaggg catgctgtgc gggcccccgc agtgctattg catcgtggag aagtggcgcg
      481 gctttgagcg catcctcccc aacaagacgc gcatcttgcg cctctgggct gcctggggcg
      541 aagagcaaga gaatgtgcgc ttcgtgctag tgcgcagcga ggcatcgctg cctaacgccg
      601 gcccccgcag cgccgaggcg cgcgtagtgc tgagccgaga gcgcccctgt ccggcccgcg
      661 gggccccggc gcggcccagc ctggccatga cccaggagaa acagcggcga gtggtgcgca
      721 aggcctttcg caaactggcc aagctcaacc ggcggcgcca gcagcagaca ccgtcgtcct
      781 gttcgtccac ttcgtcgtcc actgcctcgt cctgctcttc gtcgccgcgg acccacgaga
      841 gcgcgtcggt ggagcgcatg gagacgctgg tgcatctggt gctttcccag gaccacacaa
      901 ttcgccagca ggtgcagcgg ctccacgagc tggaccgcga gatcgatcac tacgaggcca
      961 aggtgcacct ggaccgcatg cggcgtcacg gggtcaacta cgtgcaggac acttacttgg
     1021 ttggggcagg catcgagctc gacgggtcca gaccgggaga ggagccagaa gaggtggcgg
     1081 cggaggcgga ggaggcggcg gcggcgcccc ctctagccgg cgaggcgcag gcggcggcgc
     1141 tggaggagct ggcccggcgc tgcgacgact tgctgcggct tcaggagcaa cgggttcagc
     1201 aggaggagtt gctggagcgc ctttcagccg agattcagga ggaactcaac cagaggtgga
     1261 tgcgacggcg ccaggaggag ctggcggcgc gggaggagcc cctggagccc gacggtggcc
     1321 ccgacggcga gctgctgctg gagcaggaac gggtcaggac gcagctcagt accagccttt
     1381 acattgggct gcggctcaac acggacctag aggccgtcaa gtcggacttg gattacagcc
     1441 agcagcaatg ggacagcaag aagcgcgagc tacagggcct tctgcaaact ttgcacactt
     1501 tggagctgac ggtggcaccg gatggggctc ctggctctgg cagtccctcg cgggaacctg
     1561 ggcctcaagc ctgcgccgac atgtgggtgg accaggcccg tggactggcc aagagcggtc
     1621 ctggcaacga cgaagactcg gatacgggac tgagctctat gcatagccaa gactcggact
     1681 ccttgcccat gtgcgaatcc cttgtgtaat caactttctt gtacaaagtt ggcattatag
     1741 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1801 ttatttgcat gcaagcttag atcctctaga ccagccagga cagaaatgcc tcgacttcgc
     1861 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1921 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1981 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2041 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     2101 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     2161 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     2221 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     2281 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     2341 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     2401 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     2461 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     2521 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     2581 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     2641 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2701 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2761 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2821 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2881 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2941 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3001 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3061 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3121 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3181 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3241 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3301 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3361 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3421 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     3481 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3541 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3601 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3661 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3721 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3781 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3841 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3901 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     3961 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4021 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4081 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4141 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     4201 agttccctac tctcgcg
//
