LOCUS       sequence-125987-        4214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF10 [NM_001080521.2] without stop 
            codon (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4214)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E254 Hs.RASSF10-nostop
FEATURES             Location/Qualifiers
     source          1..4214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1705..1804)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2181..2972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3065..3653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3554..3573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3807..3824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3983..4010)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4102..4188)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga tccttcggaa aagaagatat cggtgtggat ctgccaggaa gagaagctgg
      241 tgtccggcct ctcccgccgc accacttgct ccgacgttgt gcgagtgctt ttggaggacg
      301 gctgccggcg gcgacggaga cagcggcgga gccggcggct ggggtcggcc ggcgacccgc
      361 atggcccggg agagctgccc gaacccccga acgaggacga cgaggacgac gacgaggcgc
      421 tgccgcaggg catgctgtgc gggcccccgc agtgctattg catcgtggag aagtggcgcg
      481 gctttgagcg catcctcccc aacaagacgc gcatcttgcg cctctgggct gcctggggcg
      541 aagagcaaga gaatgtgcgc ttcgtgctag tgcgcagcga ggcatcgctg cctaacgccg
      601 gcccccgcag cgccgaggcg cgcgtagtgc tgagccgaga gcgcccctgt ccggcccgcg
      661 gggccccggc gcggcccagc ctggccatga cccaggagaa acagcggcga gtggtgcgca
      721 aggcctttcg caaactggcc aagctcaacc ggcggcgcca gcagcagaca ccgtcgtcct
      781 gttcgtccac ttcgtcgtcc actgcctcgt cctgctcttc gtcgccgcgg acccacgaga
      841 gcgcgtcggt ggagcgcatg gagacgctgg tgcatctggt gctttcccag gaccacacaa
      901 ttcgccagca ggtgcagcgg ctccacgagc tggaccgcga gatcgatcac tacgaggcca
      961 aggtgcacct ggaccgcatg cggcgtcacg gggtcaacta cgtgcaggac acttacttgg
     1021 ttggggcagg catcgagctc gacgggtcca gaccgggaga ggagccagaa gaggtggcgg
     1081 cggaggcgga ggaggcggcg gcggcgcccc ctctagccgg cgaggcgcag gcggcggcgc
     1141 tggaggagct ggcccggcgc tgcgacgact tgctgcggct tcaggagcaa cgggttcagc
     1201 aggaggagtt gctggagcgc ctttcagccg agattcagga ggaactcaac cagaggtgga
     1261 tgcgacggcg ccaggaggag ctggcggcgc gggaggagcc cctggagccc gacggtggcc
     1321 ccgacggcga gctgctgctg gagcaggaac gggtcaggac gcagctcagt accagccttt
     1381 acattgggct gcggctcaac acggacctag aggccgtcaa gtcggacttg gattacagcc
     1441 agcagcaatg ggacagcaag aagcgcgagc tacagggcct tctgcaaact ttgcacactt
     1501 tggagctgac ggtggcaccg gatggggctc ctggctctgg cagtccctcg cgggaacctg
     1561 ggcctcaagc ctgcgccgac atgtgggtgg accaggcccg tggactggcc aagagcggtc
     1621 ctggcaacga cgaagactcg gatacgggac tgagctctat gcatagccaa gactcggact
     1681 ccttgcccat gtgcgaatcc cttgtgtcaa ctttcttgta caaagttggc attataggaa
     1741 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1801 tttgcatgca agcttagatc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     1861 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     1921 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1981 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     2041 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     2161 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     2221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     2281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     2341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     2401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     2461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     2521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2941 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     3001 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3181 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3421 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     3481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3541 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3601 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3661 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3721 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3781 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3841 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3901 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     3961 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     4021 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     4081 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     4141 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     4201 tccctactct cgcg
//
