LOCUS       sequence-125988-        3674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF2 [NM_170774.1] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3674)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E255 Hs.RASSF2
FEATURES             Location/Qualifiers
     source          1..3674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1165..1264)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1641..2432
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2525..3113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3014..3033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3267..3284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3443..3470)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3562..3648)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga ctacagccac caaacgtccc tagtcccatg tggacaagat aaatacattt
      241 ccaaaaatga acttctcttg catctgaaga cctacaactt gtactatgaa ggccagaatt
      301 tacagctccg gcaccgggag gaagaagacg agttcattgt ggaggggctc ctgaacatct
      361 cctggggcct gcgccggccc attcgcctgc agatgcagga tgacaacgaa cgcattcgac
      421 cccctccatc ctcctcctcc tggcactctg gctgtaacct gggggctcag ggaaccactc
      481 tgaagcccct gactgtgccc aaagttcaga tctcagaggt ggatgccccg ccggagggtg
      541 accagatgcc aagctccaca gactccaggg gcctgaagcc cctgcaggag gacaccccac
      601 agctgatgcg cacacgcagt gatgttgggg tgcgtcgccg tggcaatgtg aggacgccta
      661 gtgaccagcg gcgaatcaga cgccaccgct tctccatcaa cggccatttc tacaaccata
      721 agacatccgt gttcacacca gcctatggct ctgtcaccaa cgtccgcatc aacagcacca
      781 tgaccacccc acaggtcctg aagctgctgc tcaacaaatt taagattgag aattcagcag
      841 aggagtttgc cttgtacgtg gtccatacga gtggtgagaa acagaagctg aaggccaccg
      901 attacccgct gattgcccga atcctccagg gcccatgtga gcagatctcc aaagtgttcc
      961 taatggagaa ggaccaggtg gaggaagtca cctacgacgt ggcccagtat ataaagttcg
     1021 agatgccggt acttaaaagc ttcattcaga agctccagga ggaagaagat cgggaagtaa
     1081 agaagctgat gcgcaagtac accgtgctcc ggctaatgat tcgacagagg ctggaggaga
     1141 tagccgagac cccagcaaca atctaatcaa ctttcttgta caaagttggc attataggaa
     1201 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1261 tttgcatgca agcttagatc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     1321 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     1381 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     1441 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     1501 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1561 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     1621 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     1681 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     1741 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     1801 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     1861 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     1921 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     1981 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     2041 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     2101 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     2161 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     2221 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     2281 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     2341 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     2401 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     2461 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2641 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2881 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     2941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3181 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3241 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3301 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3361 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     3421 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     3481 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     3541 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     3601 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     3661 tccctactct cgcg
//
