LOCUS       sequence-125991-        3407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF3 [NM_178169.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3407)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E258 Hs.RASSF3-nostop
FEATURES             Location/Qualifiers
     source          1..3407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(898..997)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1374..2165
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2258..2846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2747..2766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3000..3017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3176..3203)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3295..3381)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgag cagcggctac agcagcctgg aggaggacgc cgaggacttc ttcttcaccg
      241 ccaggacctc cttcttcagg agagcgcccc agggcaagcc ccgctccggc caacaagatg
      301 ttgagaaaga gaaggaaacc cacagttacc tcagcaaaga ggagatcaaa gagaaagttc
      361 ataaatacaa cttagcagtc acagacaagt tgaagatgac cttgaattca aatgggattt
      421 acactggctt cattaaagta cagatggaac tctgcaaacc tccacagact tctccaaatt
      481 ctggaaaact ctctcccagt agcaatggct gtatgaatac acttcatatc agcagcacaa
      541 acactgtcgg ggaagtgatc gaggccctgc tcaaaaagtt tctcgtgact gagagccctg
      601 ccaagtttgc actttataag cgttgtcaca gggaagacca agtctacgcc tgcaagctct
      661 cagaccggga acatccactc tacctgcgtt tggtagcagg gcccagaaca gacacactta
      721 gttttgttct tcgtgaacat gaaattggag agtgggaagc cttcagcctt ccagaactac
      781 agaatttctt gcgcatcttg gacaaggaag aagatgaaca gctgcagaac ctgaagaggc
      841 gctacacagc ctacaggcag aagctggaag aagccctccg tgaggtgtgg aagcctgatt
      901 caactttctt gtacaaagtt ggcattatag gaaagcattg cttatcaatt tgttgcaacg
      961 aacaggtcac tatcagtcaa aataaaatca ttatttgcat gcaagcttag atcctctaga
     1021 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     1081 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     1141 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1201 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     1261 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1321 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     1381 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     1441 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     1501 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     1561 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     1621 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     1681 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     1741 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     1801 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     1861 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     1921 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     1981 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     2041 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2101 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     2161 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2221 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2281 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2341 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2401 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2461 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2521 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2581 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2641 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2701 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2761 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2821 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2881 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2941 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3001 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3061 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3121 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3181 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3241 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3301 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3361 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcg
//
