LOCUS       sequence-125992-        3659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF4 [NM_032023.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3659)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E259 Hs.RASSF4
FEATURES             Location/Qualifiers
     source          1..3659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1150..1249)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1626..2417
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2510..3098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2999..3018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3252..3269
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3428..3455)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3547..3633)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgaa ggaagactgt ctgccgagtt ctcacgtgcc catcagtgac agcaagtcca
      241 ttcagaagtc ggagctctta ggcctgctga aaacctacaa ctgctaccat gagggcaaga
      301 gcttccagct gagacaccgt gaggaagaag ggactctgat catcgagggg ctcctcaaca
      361 ttgcctgggg gctgaggcgg cccatccggc tgcagatgca ggatgaccgg gagcaggtgc
      421 acctcccctc cacctcatgg atgcccagac ggcctagctg ccctctaaag gagccatcgc
      481 cccagaacgg gaacatcaca gcccaggggc caagcattca gccagtgcac aaggctgaga
      541 gttccacaga cagctcgggg cccctggagg aggcagagga ggccccccag ctgatgcgga
      601 ccaagagcga cgccagttgc atgagccaga ggaggcccaa gtgccgcgcc cccggtgagg
      661 cccagcgcat ccggcgacac cggttctcta tcaacggcca cttctacaat cataagacct
      721 ccgtgtttac tccagcctat ggatccgtga ccaatgtgag ggtcaacagc accatgacaa
      781 ccctgcaggt gctcaccctg ctgctgaaca aatttagggt ggaagatggc cccagtgagt
      841 tcgcactcta catcgttcac gagtctgggg agcggacaaa attaaaagac tgcgagtacc
      901 cgctgatttc cagaatcctg catgggccat gtgagaagat cgccaggatc ttcctgatgg
      961 aagctgactt gggcgtggaa gtcccccatg aagtcgctca gtacattaag tttgaaatgc
     1021 cggtgctgga cagttttgtt gaaaaattaa aagaagagga agaaagagaa ataatcaaac
     1081 tgaccatgaa gttccaagcc ctgcgtctga cgatgctgca gcgcctggag cagctggtgg
     1141 aggccaagta atcaactttc ttgtacaaag ttggcattat aggaaagcat tgcttatcaa
     1201 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc atgcaagctt
     1261 agatcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1321 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1381 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1441 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1501 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1561 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1621 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1681 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1741 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     1801 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1861 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1921 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1981 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2041 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2101 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2161 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2221 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2281 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2341 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2401 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2461 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2521 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2581 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2641 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2701 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2761 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2821 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2881 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2941 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3001 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3061 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3121 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3181 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3241 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3301 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3361 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3421 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3481 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3541 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3601 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcg
//
