LOCUS       sequence-125994-        3491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF5 [NM_182665.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3491)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E261 Hs.RASSF5
FEATURES             Location/Qualifiers
     source          1..3491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(982..1081)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1458..2249
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2342..2930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2831..2850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3084..3101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3260..3287)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3379..3465)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgac cgtggacagc agcatgagca gtgggtactg cagcctggac gaggaactgg
      241 aagactgctt cttcactgct aagactacct ttttcagaaa tgcgcagagc aaacatcttt
      301 caaagaatgt ctgtaaacct gtggaggaga cacagcgccc gcccacactg caggagatca
      361 agcagaagat cgacagctac aacacgcgag agaagaactg cctgggcatg aaactgagtg
      421 aagacggcac ctacacgggt ttcatcaaag tgcatctgaa actccggcgg cctgtgacgg
      481 tgcctgctgg gatccggccc cagtccatct atgatgccat caaggaggtg aacctggcgg
      541 ctaccacgga caagcggaca tccttctacc tgcccctaga tgccatcaag cagctgcaca
      601 tcagcagcac caccaccgtc agtgaggtca tccaggggct gctcaagaag ttcatggttg
      661 tggacaatcc ccagaagttt gcacttttta agcggataca caaggacgga caagtgctct
      721 tccagaaact ctccattgct gaccgccccc tctacctgcg cctgcttgct gggcctgaca
      781 cggaggtcct cagctttgtg ctaaaggaga atgaaactgg agaggtagag tgggatgcct
      841 tctccatccc tgaacttcag aacttcctaa caatcctgga aaaagaggag caggacaaaa
      901 tccaacaagt gcaaaagaag tatgacaagt ttaggcagaa actggaggag gccttaagag
      961 aatcccaggg caaacctggg taatcaactt tcttgtacaa agttggcatt ataggaaagc
     1021 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1081 gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     1141 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     1201 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     1261 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     1321 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1381 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1441 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1501 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1561 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     1621 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1681 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1741 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     1801 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     1861 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     1921 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     1981 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2041 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2101 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2161 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     2221 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     2281 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2461 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2701 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
     2761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3001 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3061 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3121 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     3181 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     3241 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     3301 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     3361 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     3421 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
     3481 ctactctcgc g
//
