LOCUS       sequence-125995-        3488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF5 [NM_182665.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3488)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E262 Hs.RASSF5-nostop
FEATURES             Location/Qualifiers
     source          1..3488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(979..1078)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1455..2246
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2339..2927
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2828..2847
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3081..3098
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3257..3284)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3376..3462)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgac cgtggacagc agcatgagca gtgggtactg cagcctggac gaggaactgg
      241 aagactgctt cttcactgct aagactacct ttttcagaaa tgcgcagagc aaacatcttt
      301 caaagaatgt ctgtaaacct gtggaggaga cacagcgccc gcccacactg caggagatca
      361 agcagaagat cgacagctac aacacgcgag agaagaactg cctgggcatg aaactgagtg
      421 aagacggcac ctacacgggt ttcatcaaag tgcatctgaa actccggcgg cctgtgacgg
      481 tgcctgctgg gatccggccc cagtccatct atgatgccat caaggaggtg aacctggcgg
      541 ctaccacgga caagcggaca tccttctacc tgcccctaga tgccatcaag cagctgcaca
      601 tcagcagcac caccaccgtc agtgaggtca tccaggggct gctcaagaag ttcatggttg
      661 tggacaatcc ccagaagttt gcacttttta agcggataca caaggacgga caagtgctct
      721 tccagaaact ctccattgct gaccgccccc tctacctgcg cctgcttgct gggcctgaca
      781 cggaggtcct cagctttgtg ctaaaggaga atgaaactgg agaggtagag tgggatgcct
      841 tctccatccc tgaacttcag aacttcctaa caatcctgga aaaagaggag caggacaaaa
      901 tccaacaagt gcaaaagaag tatgacaagt ttaggcagaa actggaggag gccttaagag
      961 aatcccaggg caaacctggg tcaactttct tgtacaaagt tggcattata ggaaagcatt
     1021 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     1081 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1141 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     1201 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     1261 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1321 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1381 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1441 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1501 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1561 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     1621 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1681 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     1741 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     1801 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     1861 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     1921 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     1981 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2041 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2101 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2161 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     2221 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     2281 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2461 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     2521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2701 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     2761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2821 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2881 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3001 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3061 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3121 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3181 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3241 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     3301 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     3361 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3421 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     3481 ctctcgcg
//
