LOCUS       sequence-125997-        3704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF6 [NM_177532.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3704)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E264 Hs.RASSF6-nostop
FEATURES             Location/Qualifiers
     source          1..3704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1195..1294)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1671..2462
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2555..3143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3044..3063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3297..3314
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3473..3500)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3592..3678)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgac tatgatggct caccagtacc cctcttggat cttcattaat gagaagacat
      241 tcataaccag ggaacaactt aattctttat tgaagaccta taacattttt tatgagaacc
      301 agaaaaatct gcatatttta tatggagaga ctgaagatgg caaactaatt gttgaaggaa
      361 tgctggacat tttctgggga gtaaaacgac ctatacagct aaaaatacaa gatgagaagc
      421 cattctcttc ttttactagt atgaagtcat cagacgtctt ctccagcaaa ggaatgacac
      481 gctgggggga atttgacgat ctctatcgta ttagtgagct ggacaggacc cagattccta
      541 tgtctgaaaa aaggaattcc caggaagact atttatctta tcacagcaac accctgaagc
      601 cacatgcaaa ggatgaacca gactccccag tgctctatag aaccatgagt gaagcagctc
      661 tggtgagaaa aaggatgaag cctctgatga tggacagaaa agaaagacag aaaaatagag
      721 cctctattaa tggacacttc tataaccatg aaacatcaat tttcattcca gcctttgaat
      781 cagaaactaa ggtcagagta aacagtaaca tgagaactga agaagtaata aagcaacttc
      841 tccaaaaatt taagattgaa aatagtcccc aggattttgc tcttcacatt atttttgcaa
      901 caggagaaca aagacgacta aagaagacag acattccgct actgcagagg ctcctacagg
      961 gaccttctga aaagaatgct cgcattttcc tcatggataa agatgcagaa gaaattagca
     1021 gtgatgtggc tcagtacatt aactttcact tttctctctt ggaatccatt cttcaaagat
     1081 taaatgaaga agagaaaaga gagattcaaa gaatagtaac aaaattcaat aaagaaaagg
     1141 cgattatact gaaatgtctt caaaataaac tagtaataaa aacagagaca acagtttcaa
     1201 ctttcttgta caaagttggc attataggaa agcattgctt atcaatttgt tgcaacgaac
     1261 aggtcactat cagtcaaaat aaaatcatta tttgcatgca agcttagatc ctctagacca
     1321 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1381 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1441 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1501 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     1561 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     1621 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     1681 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     1741 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     1801 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     1861 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     1921 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     1981 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2041 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2101 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2161 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2221 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2281 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2341 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2401 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2461 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2521 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2581 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2641 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2701 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2761 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2821 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2881 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2941 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3001 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3061 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3121 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3181 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3241 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3301 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3361 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3421 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3481 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3541 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     3601 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     3661 agcctttcgt tttatttgat gcctggcagt tccctactct cgcg
//
