LOCUS       sequence-126-exp        4828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2680
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             576..596
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(639..667)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1415..1435
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2402..2424)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2524..2543
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2621..2639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2707..2811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2812..2880,2881..3672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3030..3049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3843..4431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4332..4351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4585..4602
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4719..4740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4755..4760,4761..4778,4779..4785)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4793..4809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4798..4820
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4817..4828,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4817..4828,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tcaagagcat
       61 ttgaatcaga atatggagaa cggagcatga gcattttcga agttttttag atgcactaga
      121 acaaagcgtg ttggcttcct ctgagcccgc tttccttata tacccgcatt ctgcagcctt
      181 acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa atactcccgg tgggtgcaaa
      241 gagacgcaga cggaaaatgt atctgggtct ctttattgtg tacactactt ttccatgtac
      301 cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa tgttctagaa aaaggtggaa
      361 aatagtataa ataccgttga aaataaatac cgaacaacat ttgctctaat tgtgaaatta
      421 gaaatcttca aactataatc atctcactgg atccccggga ttggccaaag gacccaaagg
      481 tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct tggctagagg
      541 atccccgggt accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
      601 tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      661 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      721 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg
      781 taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg
      841 tcactacttt ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc
      901 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
      961 aagatgacgg gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca
     1021 tatttaaatt ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1081 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1141 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg
     1201 taagtttaaa catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa
     1261 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1321 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1381 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1441 ctgggattac acatggcatg gatgaactat acaaatagca ttcgtagaat tccaactgag
     1501 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc
     1561 agagtaagtt taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc
     1621 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     1681 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     1741 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     1801 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     1861 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     1921 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     1981 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     2041 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     2101 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     2161 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     2221 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     2281 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     2341 ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     2401 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     2461 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     2521 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     2581 gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt
     2641 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     2701 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2761 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2821 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2881 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2941 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3001 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3061 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3121 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3181 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3241 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3301 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     3361 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     3421 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     3481 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     3541 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     3601 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3661 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3721 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3781 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3841 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3901 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3961 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     4021 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4081 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4141 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4201 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     4261 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4321 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4381 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4441 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4501 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4561 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     4621 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4681 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     4741 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     4801 ggataacaat ttcacacagg aaacagct
//
