LOCUS       sequence-126002-        4001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF9 [NM_005447.3] with stop codon (for
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4001)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E269 Hs.RASSF9
FEATURES             Location/Qualifiers
     source          1..4001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1492..1591)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1968..2759
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2852..3440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3341..3360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3594..3611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3770..3797)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3889..3975)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc tccctttgga agaaacttgc taaagactcg gcataaaaac agatctccaa
      241 ctaaagacat ggattcagaa gagaaggaaa ttgtggtttg ggtttgccaa gaagagaagc
      301 ttgtctgtgg gctgactaaa cgcaccacct ctgctgatgt catccaggct ttgcttgagg
      361 aacatgaggc tacgtttgga gagaaacgat ttcttctggg gaagcccagt gattactgca
      421 tcatagagaa gtggagaggc tccgaaaggg ttcttcctcc actaactaga atcctgaagc
      481 tttggaaagc gtggggagat gagcagccca atatgcaatt tgttttggtt aaagcagatg
      541 cttttcttcc agttcctttg tggcggacag ctgaagccaa attagtgcaa aacacagaaa
      601 aattgtggga gctcagccca gcaaactaca tgaagacttt accaccagat aaacaaaaaa
      661 gaatagtcag gaaaactttc cggaaactgg ctaaaattaa gcaggacaca gtttctcatg
      721 atcgagataa tatggagaca ttagttcatc tgatcatttc ccaggaccat actattcatc
      781 agcaagtcaa gagaatgaaa gagctggatc tggaaattga aaagtgtgaa gctaagttcc
      841 atcttgatcg agtagaaaat gatggagaaa actatgttca ggatgcatat ttaatgccca
      901 gtttcagtga agttgagcaa aatctagact tgcagtatga ggaaaaccag actctggagg
      961 acctgagcga aagtgatgga attgaacagc tggaagaacg actgaaatat taccgaatac
     1021 tcattgataa gctctctgct gaaatagaaa aagaggtaaa aagtgtttgc attgatataa
     1081 atgaagatgc ggaaggggaa gctgcaagtg aactggaaag ctctaattta gagagtgtta
     1141 agtgtgattt ggagaaaagc atgaaagctg gtttgaaaat tcactctcat ttgagtggca
     1201 tccagaaaga gattaaatac agtgactcat tgcttcagat gaaagcaaaa gaatatgaac
     1261 tcctggccaa ggaattcaat tcacttcaca ttagcaacaa agatgggtgc cagttaaagg
     1321 aaaacagagc gaaggaatct gaggttccca gtagcaatgg ggagattcct ccctttactc
     1381 aaagagtatt tagcaattac acaaatgaca cagactcgga cactggtatc agttctaacc
     1441 acagtcagga ctccgaaaca acagtaggag atgtggtgct gttgtcaaca taatcaactt
     1501 tcttgtacaa agttggcatt ataggaaagc attgcttatc aatttgttgc aacgaacagg
     1561 tcactatcag tcaaaataaa atcattattt gcatgcaagc ttagatcctc tagaccagcc
     1621 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     1681 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     1741 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1801 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     1861 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     1921 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     1981 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2041 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2101 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2161 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2221 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2281 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2341 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2401 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2461 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2521 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2581 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     2641 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     2701 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     2761 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     2821 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2881 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2941 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3001 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3061 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3121 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3181 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3241 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3301 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3361 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3421 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3481 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3541 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3601 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3661 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3721 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     3781 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     3841 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     3901 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     3961 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc g
//
