LOCUS       sequence-126003-        3998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RASSF9 [NM_005447.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3998)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E270 Hs.RASSF9-nostop
FEATURES             Location/Qualifiers
     source          1..3998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1489..1588)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1965..2756
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2849..3437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3338..3357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3591..3608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3767..3794)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3886..3972)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc tccctttgga agaaacttgc taaagactcg gcataaaaac agatctccaa
      241 ctaaagacat ggattcagaa gagaaggaaa ttgtggtttg ggtttgccaa gaagagaagc
      301 ttgtctgtgg gctgactaaa cgcaccacct ctgctgatgt catccaggct ttgcttgagg
      361 aacatgaggc tacgtttgga gagaaacgat ttcttctggg gaagcccagt gattactgca
      421 tcatagagaa gtggagaggc tccgaaaggg ttcttcctcc actaactaga atcctgaagc
      481 tttggaaagc gtggggagat gagcagccca atatgcaatt tgttttggtt aaagcagatg
      541 cttttcttcc agttcctttg tggcggacag ctgaagccaa attagtgcaa aacacagaaa
      601 aattgtggga gctcagccca gcaaactaca tgaagacttt accaccagat aaacaaaaaa
      661 gaatagtcag gaaaactttc cggaaactgg ctaaaattaa gcaggacaca gtttctcatg
      721 atcgagataa tatggagaca ttagttcatc tgatcatttc ccaggaccat actattcatc
      781 agcaagtcaa gagaatgaaa gagctggatc tggaaattga aaagtgtgaa gctaagttcc
      841 atcttgatcg agtagaaaat gatggagaaa actatgttca ggatgcatat ttaatgccca
      901 gtttcagtga agttgagcaa aatctagact tgcagtatga ggaaaaccag actctggagg
      961 acctgagcga aagtgatgga attgaacagc tggaagaacg actgaaatat taccgaatac
     1021 tcattgataa gctctctgct gaaatagaaa aagaggtaaa aagtgtttgc attgatataa
     1081 atgaagatgc ggaaggggaa gctgcaagtg aactggaaag ctctaattta gagagtgtta
     1141 agtgtgattt ggagaaaagc atgaaagctg gtttgaaaat tcactctcat ttgagtggca
     1201 tccagaaaga gattaaatac agtgactcat tgcttcagat gaaagcaaaa gaatatgaac
     1261 tcctggccaa ggaattcaat tcacttcaca ttagcaacaa agatgggtgc cagttaaagg
     1321 aaaacagagc gaaggaatct gaggttccca gtagcaatgg ggagattcct ccctttactc
     1381 aaagagtatt tagcaattac acaaatgaca cagactcgga cactggtatc agttctaacc
     1441 acagtcagga ctccgaaaca acagtaggag atgtggtgct gttgtcaaca tcaactttct
     1501 tgtacaaagt tggcattata ggaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1561 ctatcagtca aaataaaatc attatttgca tgcaagctta gatcctctag accagccagg
     1621 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     1681 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1741 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1801 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     1861 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1921 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     1981 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2041 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2101 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2161 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2221 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2281 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2341 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2401 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2461 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2521 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2581 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2641 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2701 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     2761 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2821 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2881 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2941 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3001 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3061 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3121 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3181 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3241 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3301 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3361 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3421 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3481 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3541 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3601 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3661 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3721 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3781 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3841 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3901 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3961 tcgttttatt tgatgcctgg cagttcccta ctctcgcg
//
