LOCUS       sequence-126005-        5477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RB1 [NM_000321.2] without stop codon (for
            C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5477)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E272 Hs.RB1-nostop
FEATURES             Location/Qualifiers
     source          1..5477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2968..3067)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3444..4235
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4328..4916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4817..4836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5070..5087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5246..5273)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5365..5451)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc gcccaaaacc ccccgaaaaa cggccgccac cgccgccgct gccgccgcgg
      241 aacccccggc accgccgccg ccgccccctc ctgaggagga cccagagcag gacagcggcc
      301 cggaggacct gcctctcgtc aggcttgagt ttgaagaaac agaagaacct gattttactg
      361 cattatgtca gaaattaaag ataccagatc atgtcagaga gagagcttgg ttaacttggg
      421 agaaagtttc atctgtggat ggagtattgg gaggttatat tcaaaagaaa aaggaactgt
      481 ggggaatctg tatctttatt gcagcagttg acctagatga gatgtcgttc acttttactg
      541 agctacagaa aaacatagaa atcagtgtcc ataaattctt taacttacta aaagaaattg
      601 ataccagtac caaagttgat aatgctatgt caagactgtt gaagaagtat gatgtattgt
      661 ttgcactctt cagcaaattg gaaaggacat gtgaacttat atatttgaca caacccagca
      721 gttcgatatc tactgaaata aattctgcat tggtgctaaa agtttcttgg atcacatttt
      781 tattagctaa aggggaagta ttacaaatgg aagatgatct ggtgatttca tttcagttaa
      841 tgctatgtgt ccttgactat tttattaaac tctcacctcc catgttgctc aaagaaccat
      901 ataaaacagc tgttataccc attaatggtt cacctcgaac acccaggcga ggtcagaaca
      961 ggagtgcacg gatagcaaaa caactagaaa atgatacaag aattattgaa gttctctgta
     1021 aagaacatga atgtaatata gatgaggtga aaaatgttta tttcaaaaat tttatacctt
     1081 ttatgaattc tcttggactt gtaacatcta atggacttcc agaggttgaa aatctttcta
     1141 aacgatacga agaaatttat cttaaaaata aagatctaga tgcaagatta tttttggatc
     1201 atgataaaac tcttcagact gattctatag acagttttga aacacagaga acaccacgaa
     1261 aaagtaacct tgatgaagag gtgaatgtaa ttcctccaca cactccagtt aggactgtta
     1321 tgaacactat ccaacaatta atgatgattt taaattcagc aagtgatcaa ccttcagaaa
     1381 atctgatttc ctattttaac aactgcacag tgaatccaaa agaaagtata ctgaaaagag
     1441 tgaaggatat aggatacatc tttaaagaga aatttgctaa agctgtggga cagggttgtg
     1501 tcgaaattgg atcacagcga tacaaacttg gagttcgctt gtattaccga gtaatggaat
     1561 ccatgcttaa atcagaagaa gaacgattat ccattcaaaa ttttagcaaa cttctgaatg
     1621 acaacatttt tcatatgtct ttattggcgt gcgctcttga ggttgtaatg gccacatata
     1681 gcagaagtac atctcagaat cttgattctg gaacagattt gtctttccca tggattctga
     1741 atgtgcttaa tttaaaagcc tttgattttt acaaagtgat cgaaagtttt atcaaagcag
     1801 aaggcaactt gacaagagaa atgataaaac atttagaacg atgtgaacat cgaatcatgg
     1861 aatcccttgc atggctctca gattcacctt tatttgatct tattaaacaa tcaaaggacc
     1921 gagaaggacc aactgatcac cttgaatctg cttgtcctct taatcttcct ctccagaata
     1981 atcacactgc agcagatatg tatctttctc ctgtaagatc tccaaagaaa aaaggttcaa
     2041 ctacgcgtgt aaattctact gcaaatgcag agacacaagc aacctcagcc ttccagaccc
     2101 agaagccatt gaaatctacc tctctttcac tgttttataa aaaagtgtat cggctagcct
     2161 atctccggct aaatacactt tgtgaacgcc ttctgtctga gcacccagaa ttagaacata
     2221 tcatctggac ccttttccag cacaccctgc agaatgagta tgaactcatg agagacaggc
     2281 atttggacca aattatgatg tgttccatgt atggcatatg caaagtgaag aatatagacc
     2341 ttaaattcaa aatcattgta acagcataca aggatcttcc tcatgctgtt caggagacat
     2401 tcaaacgtgt tttgatcaaa gaagaggagt atgattctat tatagtattc tataactcgg
     2461 tcttcatgca gagactgaaa acaaatattt tgcagtatgc ttccaccagg ccccctacct
     2521 tgtcaccaat acctcacatt cctcgaagcc cttacaagtt tcctagttca cccttacgga
     2581 ttcctggagg gaacatctat atttcacccc tgaagagtcc atataaaatt tcagaaggtc
     2641 tgccaacacc aacaaaaatg actccaagat caagaatctt agtatcaatt ggtgaatcat
     2701 tcgggacttc tgagaagttc cagaaaataa atcagatggt atgtaacagc gaccgtgtgc
     2761 tcaaaagaag tgctgaagga agcaaccctc ctaaaccact gaaaaaacta cgctttgata
     2821 ttgaaggatc agatgaagca gatggaagta aacatctccc aggagagtcc aaatttcagc
     2881 agaaactggc agaaatgact tctactcgaa cacgaatgca aaagcagaaa atgaatgata
     2941 gcatggatac ctcaaacaag gaagagaaat caactttctt gtacaaagtt ggcattatag
     3001 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     3061 ttatttgcat gcaagcttag atcctctaga ccagccagga cagaaatgcc tcgacttcgc
     3121 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     3181 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     3241 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     3301 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     3361 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     3421 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     3481 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3541 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3601 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3661 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3721 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3781 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3841 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3901 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3961 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     4021 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     4081 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     4141 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     4201 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     4261 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4441 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4681 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5161 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     5221 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     5281 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     5341 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     5401 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     5461 agttccctac tctcgcg
//
