LOCUS       sequence-126013-        3281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RHOB [NM_004040.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3281)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E280 Hs.RHOB-nostop
FEATURES             Location/Qualifiers
     source          1..3281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      184..193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(772..871)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1248..2039
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2132..2720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2621..2640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2874..2891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3050..3077)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3169..3255)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatggc ggccatccgc aagaagctgg tggtggtggg cgacggcgcg tgtggcaaga
      241 cgtgcctgct gatcgtgttc agtaaggacg agttccccga ggtgtacgtg cccaccgtct
      301 tcgagaacta tgtggccgac attgaggtgg acggcaagca ggtggagctg gcgctgtggg
      361 acacggcggg ccaggaggac tacgaccgcc tgcggccgct ctcctacccg gacaccgacg
      421 tcattctcat gtgcttctcg gtggacagcc cggactcgct ggagaacatc cccgagaagt
      481 gggtccccga ggtgaagcac ttctgtccca atgtgcccat catcctggtg gccaacaaaa
      541 aagacctgcg cagcgacgag catgtccgca cagagctggc ccgcatgaag caggaacccg
      601 tgcgcacgga tgacggccgc gccatggccg tgcgcatcca agcctacgac tacctcgagt
      661 gctctgccaa gaccaaggaa ggcgtgcgcg aggtcttcga gacggccacg cgcgccgcgc
      721 tgcagaagcg ctacggctcc cagaacggct gcatcaactg ctgcaaggtg ctatcaactt
      781 tcttgtacaa agttggcatt ataggaaagc attgcttatc aatttgttgc aacgaacagg
      841 tcactatcag tcaaaataaa atcattattt gcatgcaagc ttagatcctc tagaccagcc
      901 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
      961 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     1021 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1081 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     1141 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     1201 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     1261 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     1321 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     1381 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     1441 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     1501 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     1561 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     1621 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     1681 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     1741 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     1801 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     1861 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     1921 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     1981 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     2041 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     2101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2521 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2881 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2941 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3001 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     3061 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     3121 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     3181 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     3241 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc g
//
