LOCUS       sequence-126023-        4898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RPS6KA1 [NM_002953.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4898)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E290 Hs.RPS6KA1-nostop
FEATURES             Location/Qualifiers
     source          1..4898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2389..2488)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2865..3656
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3749..4337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4238..4257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4491..4508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4667..4694)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4786..4872)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc gctcgcccag ctcaaggagc cctggccgct catggagcta gtgccgctgg
      241 acccggagaa tggacagacc tcaggggaag aagctggact tcagccgtcc aaggatgagg
      301 gcgtcctcaa ggagatctcc atcacgcacc acgtcaaggc tggctctgag aaggctgatc
      361 catcccattt cgagctcctc aaggttctgg gccagggatc ctttggcaaa gtcttcctgg
      421 tgcggaaagt cacccggcct gacagtgggc acctgtatgc tatgaaggtg ctgaagaagg
      481 caacgctgaa agtacgtgac cgcgtccgga ccaagatgga gagagacatc ctggctgatg
      541 taaatcaccc attcgtggtg aagctgcact atgccttcca gaccgagggc aagctctatc
      601 tcattctgga cttcctgcgt ggtggggacc tcttcacccg gctctcaaaa gaggtgatgt
      661 tcacggagga ggatgtgaag ttttacctgg ccgagctggc tctgggcctg gatcacctgc
      721 acagcctggg tatcatttac agagacctca agcctgagaa catccttctg gatgaggagg
      781 gccacatcaa actcactgac tttggcctga gcaaagaggc cattgaccac gagaagaagg
      841 cctattcttt ctgcgggaca gtggagtaca tggcccctga ggtcgtcaac cgccagggcc
      901 actcccatag tgcggactgg tggtcctatg gggtgttgat gtttgagatg ctgacgggct
      961 ccctgccctt ccaggggaag gaccggaagg agaccatgac actgattctg aaggcgaagc
     1021 taggcatgcc ccagtttctg agcactgaag cccagagcct cttgcgggcc ctgttcaagc
     1081 ggaatcctgc caaccggctc ggctccggcc ctgatggggc agaggaaatc aagcggcatg
     1141 tcttctactc caccattgac tggaataagc tataccgtcg tgagatcaag ccacccttca
     1201 agccagcagt ggctcagcct gatgacacct tctactttga caccgagttc acgtcccgca
     1261 cacccaagga ttccccaggc atccccccca gcgctggggc ccatcagctg ttccggggct
     1321 tcagcttcgt ggccaccggc ctgatggaag acgacggcaa gcctcgtgcc ccgcaggcac
     1381 ccctgcactc ggtggtacag caactccatg ggaagaacct ggtttttagt gacggctacg
     1441 tggtaaagga gacaattggt gtgggctcct actctgagtg caagcgctgt gtccacaagg
     1501 ccaccaacat ggagtatgct gtcaaggtca ttgataagag caagcgggat ccttcagaag
     1561 agattgagat tcttctgcgg tatggccagc accccaacat catcactctg aaagatgtgt
     1621 atgatgatgg caaacacgtg tacctggtga cagagctgat gcggggtggg gagctgctgg
     1681 acaagatcct gcggcagaag ttcttctcag agcgggaggc cagctttgtc ctgcacacca
     1741 ttggcaaaac tgtggagtat ctgcactcac agggggttgt gcacagggac ctgaagccca
     1801 gcaacatcct gtatgtggac gagtccggga atcccgagtg cctgcgcatc tgtgactttg
     1861 gttttgccaa acagctgcgg gctgagaatg ggctcctcat gacaccttgc tacacagcca
     1921 actttgtggc gcctgaggtg ctgaagcgcc agggctacga tgaaggctgc gacatctgga
     1981 gcctgggcat tctgctgtac accatgctgg caggatatac tccatttgcc aacggtccca
     2041 gtgacacacc agaggaaatc ctaacccgga tcggcagtgg gaagtttacc ctcagtgggg
     2101 gaaattggaa cacagtttca gagacagcca aggacctggt gtccaagatg ctacacgtgg
     2161 atccccacca gcgcctcaca gctaagcagg ttctgcagca tccatgggtc acccagaaag
     2221 acaagcttcc ccaaagccag ctgtcccacc aggacctaca gcttgtgaag ggagccatgg
     2281 ctgccacgta ctccgcactc aacagctcca agcccacccc ccagctgaag cccatcgagt
     2341 catccatcct ggcccagcgg cgagtgagga agttgccatc caccaccctg tcaactttct
     2401 tgtacaaagt tggcattata ggaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     2461 ctatcagtca aaataaaatc attatttgca tgcaagctta gatcctctag accagccagg
     2521 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2581 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2641 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2701 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2761 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2821 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2881 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2941 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3001 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3061 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3121 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3181 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3241 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3301 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3361 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3421 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3481 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3541 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3601 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3661 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4141 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4381 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4441 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4501 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4561 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4621 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     4681 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     4741 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4801 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4861 tcgttttatt tgatgcctgg cagttcccta ctctcgcg
//
