LOCUS       sequence-126027-        4913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human RPS6KA3 [NM_004586.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4913)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E294 Hs.RPS6KA3-nostop
FEATURES             Location/Qualifiers
     source          1..4913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2404..2503)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2880..3671
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3764..4352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4253..4272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4506..4523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4682..4709)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4801..4887)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgcc gctggcgcag ctggcggacc cgtggcagaa gatggctgtg gagagcccgt
      241 ccgacagcgc tgagaatgga cagcaaatta tggatgaacc tatgggagag gaggagatta
      301 acccacaaac tgaagaagtc agtatcaaag aaattgcaat cacacatcat gtaaaggaag
      361 gacatgaaaa ggcagatcct tcccagtttg aacttttaaa agtattaggg cagggatcat
      421 ttggaaaggt tttcttagtt aaaaaaatct caggctctga tgctaggcag ctttatgcca
      481 tgaaggtatt gaagaaggcc acactgaaag ttcgagaccg agttcggaca aaaatggaac
      541 gtgatatctt ggtagaggtt aatcatcctt ttattgtcaa gttgcattat gcttttcaaa
      601 ctgaagggaa gttgtatctt attttggatt ttctcagggg aggagatttg tttacacgct
      661 tatccaaaga ggtgatgttc acagaagaag atgtcaaatt ctacttggct gaacttgcac
      721 ttgctttaga ccatctacat agcctgggaa taatttatag agacttaaaa ccagaaaata
      781 tacttcttga tgaagaaggt cacatcaagt taacagattt cggcctaagt aaagagtcta
      841 ttgaccatga aaagaaggca tattcttttt gtggaactgt ggagtatatg gctccagaag
      901 tagttaatcg tcgaggtcat actcagagtg ctgactggtg gtcttttggt gtgttaatgt
      961 ttgaaatgct tactggtaca ctccctttcc aaggaaaaga tcgaaaagaa acaatgacta
     1021 tgattcttaa agccaaactt ggaatgccac agtttttgag tcctgaagcg cagagtcttt
     1081 tacgaatgct tttcaagcga aatcctgcaa acagattagg tgcaggacca gatggagttg
     1141 aagaaattaa aagacattca tttttctcaa cgatagactg gaataaactg tatagaagag
     1201 aaattcatcc gccatttaaa cctgcaacgg gcaggcctga agatacattc tattttgatc
     1261 ctgagtttac tgcaaaaact cccaaagatt cacctggcat tccacctagt gctaatgcac
     1321 atcagctttt tcgggggttt agttttgttg ctattacctc agatgatgaa agccaagcta
     1381 tgcagacagt tggtgtacat tcaattgttc agcagttaca caggaacagt attcagttta
     1441 ctgatggata tgaagtaaaa gaagatattg gagttggctc ctactctgtt tgcaagagat
     1501 gtatacataa agctacaaac atggagtttg cagtgaagat tattgataaa agcaagagag
     1561 acccaacaga agaaattgaa attcttcttc gttatggaca gcatccaaac attatcactc
     1621 taaaggatgt atatgatgat ggaaagtatg tgtatgtagt aacagaactt atgaaaggag
     1681 gtgaattgct ggataaaatt cttagacaaa aatttttctc tgaacgagag gccagtgctg
     1741 tcctgttcac tataactaaa accgttgaat atcttcacgc acaaggggtg gttcatagag
     1801 acttgaaacc tagcaacatt ctttatgtgg atgaatctgg taatccggaa tctattcgaa
     1861 tttgtgattt tggctttgca aaacagctga gagcggaaaa tggtcttctc atgactcctt
     1921 gttacactgc aaattttgtt gcaccagagg ttttaaaaag acaaggctat gatgctgctt
     1981 gtgatatatg gagtcttggt gtcctactct atacaatgct taccggttac actccatttg
     2041 caaatggtcc tgatgataca ccagaggaaa tattggcacg aataggtagc ggaaaattct
     2101 cactcagtgg tggttactgg aattctgttt cagacacagc aaaggacctg gtgtcaaaga
     2161 tgcttcatgt agaccctcat cagagactga ctgctgctct tgtgctcaga catccttgga
     2221 tcgtccactg ggaccaactg ccacaatacc aactaaacag acaggatgca ccacatctag
     2281 taaagggtgc catggcagct acatattctg ctttgaaccg taatcagtca ccagttttgg
     2341 aaccagtagg ccgctctact cttgctcagc ggagaggtat taaaaaaatc acctcaacag
     2401 ccctgtcaac tttcttgtac aaagttggca ttataggaaa gcattgctta tcaatttgtt
     2461 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgcatgcaa gcttagatcc
     2521 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac
     2581 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     2641 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     2701 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     2761 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     2821 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta
     2881 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     2941 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     3001 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     3061 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     3121 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     3181 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     3241 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     3301 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     3361 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     3421 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     3481 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     3541 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     3601 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     3661 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt
     3721 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3781 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3841 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3901 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3961 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4021 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4081 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4141 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4201 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4261 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4321 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4381 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4441 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4501 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4561 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4621 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     4681 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     4741 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg
     4801 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     4861 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcg
//
