LOCUS       sequence-126037-        3842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human SAV1 [NM_021818.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3842)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E304 Hs.SAV1-nostop
FEATURES             Location/Qualifiers
     source          1..3842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1333..1432)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1809..2600
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2693..3281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3182..3201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3435..3452
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3611..3638)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3730..3816)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgct gtcccgaaag aaaaccaaaa acgaagtgtc caagccggcc gaggtgcagg
      241 ggaagtacgt gaagaaggag acgtcgcctc tgcttcggaa tcttatgcct tcattcatcc
      301 ggcatggtcc aacaattcca agacgaactg atatctgtct tccagattca agccctaatg
      361 ccttttcaac ttctggagat gtagtttcaa gaaaccagag tttccttaga actccaattc
      421 aaagaacacc tcatgaaata atgagaagag aaagcaacag attatctgca ccttcttatc
      481 ttgccagaag tctagcagat gtccctagag agtatggttc ttctcagtca tttgtaacgg
      541 aagttagttt tgctgttgaa aatggagact ctggttcccg atattattat tcagacaatt
      601 tttttgatgg tcagagaaag cggccacttg gagatcgtgc acatgaagac tacagatatt
      661 atgaatacaa ccatgatctc ttccaaagaa tgccacagaa tcaggggagg catgcttcag
      721 gtattgggag agttgctgct acatctttag gaaatttgac taaccatggt tctgaagatt
      781 taccccttcc tcctggctgg tctgtggact ggacaatgag agggagaaaa tattatatag
      841 atcataacac aaatacaact cactggagcc atcctcttga gcgagaagga cttcctcctg
      901 gatgggaacg agttgagtca tccgaatttg gaacctatta tgtagatcac acaaataaga
      961 aggcccaata caggcatccc tgtgctccta gtgtacctcg gtatgatcaa ccacctcctg
     1021 tcacatacca gccacagcaa actgaaagaa atcagtccct tctggtacct gcaaatccat
     1081 atcatactgc agaaattcct gactggcttc aggtttacgc acgagcccct gtgaaatatg
     1141 accacattct gaagtgggaa ctcttccagc tggctgacct ggatacatac cagggaatgc
     1201 taaagttgct cttcatgaaa gaattggagc agattgttaa aatgtatgaa gcatacagac
     1261 aagcccttct tacagagttg gaaaaccgaa agcagagaca gcagtggtat gcccaacaac
     1321 atggaaaaaa tttttcaact ttcttgtaca aagttggcat tataggaaag cattgcttat
     1381 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgcatgcaag
     1441 cttagatcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     1501 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     1561 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     1621 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1681 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     1741 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     1801 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     1861 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     1921 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     1981 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2041 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2101 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2161 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2221 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2281 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2341 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     2401 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     2461 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     2521 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     2581 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     2641 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2701 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2761 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2821 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2881 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2941 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3001 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3061 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
     3121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3361 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3421 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3481 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3541 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     3601 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     3661 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     3721 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     3781 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     3841 cg
//
