LOCUS       sequence-126040-        4118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human SHC1 [NM_003029.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4118)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E307 Hs.SHC1
FEATURES             Location/Qualifiers
     source          1..4118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1609..1708)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2085..2876
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2969..3557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3458..3477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3711..3728
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3887..3914)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4006..4092)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgaa caagctgagt ggaggcggcg ggcgcaggac tcgggtggaa gggggccagc
      241 ttgggggcga ggagtggacc cgccacggga gctttgtcaa taagcccacg cggggctggc
      301 tgcatcccaa cgacaaagtc atgggacccg gggtttccta cttggttcgg tacatgggtt
      361 gtgtggaggt cctccagtca atgcgtgccc tggacttcaa cacccggact caggtcacca
      421 gggaggccat cagtctggtg tgtgaggctg tgccgggtgc taagggggcg acaaggagga
      481 gaaagccctg tagccgcccg ctcagctcta tcctggggag gagtaacctg aaatttgctg
      541 gaatgccaat cactctcacc gtctccacca gcagcctcaa cctcatggcc gcagactgca
      601 aacagatcat cgccaaccac cacatgcaat ctatctcatt tgcatccggc ggggatccgg
      661 acacagccga gtatgtcgcc tatgttgcca aagaccctgt gaatcagaga gcctgccaca
      721 ttctggagtg tcccgaaggg cttgcccagg atgtcatcag caccattggc caggccttcg
      781 agttgcgctt caaacaatac ctcaggaacc cacccaaact ggtcacccct catgacagga
      841 tggctggctt tgatggctca gcatgggatg aggaggagga agagccacct gaccatcagt
      901 actataatga cttcccgggg aaggaacccc ccttgggggg ggtggtagac atgaggcttc
      961 gggaaggagc cgctccaggg gctgctcgac ccactgcacc caatgcccag acccccagcc
     1021 acttgggagc tacattgcct gtaggacagc ctgttggggg agatccagaa gtccgcaaac
     1081 agatgccacc tccaccaccc tgtccagcag gcagagagct ttttgatgat ccctcctatg
     1141 tcaacgtcca gaacctagac aaggcccggc aagcagtggg tggtgctggg ccccccaatc
     1201 ctgctatcaa tggcagtgca ccccgggacc tgtttgacat gaagcccttc gaagatgctc
     1261 ttcgcgtgcc tccacctccc cagtcggtgt ccatggctga gcagctccga ggggagccct
     1321 ggttccatgg gaagctgagc cggcgggagg ctgaggcact gctgcagctc aatggggact
     1381 tcctggtacg ggagagcacg accacacctg gccagtatgt gctcactggc ttgcagagtg
     1441 ggcagcctaa gcatttgcta ctggtggacc ctgagggtgt ggttcggact aaggatcacc
     1501 gctttgaaag tgtcagtcac cttatcagct accacatgga caatcacttg cccatcatct
     1561 ctgcgggcag cgaactgtgt ctacagcaac ctgtggagcg gaaactgtaa tcaactttct
     1621 tgtacaaagt tggcattata ggaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1681 ctatcagtca aaataaaatc attatttgca tgcaagctta gatcctctag accagccagg
     1741 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     1801 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1861 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1921 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     1981 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2041 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2101 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2161 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2221 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2281 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2341 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2401 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2461 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2521 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2581 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2641 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2701 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2761 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2821 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     2881 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2941 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3001 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3061 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3361 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3601 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3661 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3721 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3781 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3841 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3901 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3961 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4021 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4081 tcgttttatt tgatgcctgg cagttcccta ctctcgcg
//
