LOCUS       sequence-126057-        3947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human SPRED2 [NM_181784.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3947)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E324 Hs.SPRED2-nostop
FEATURES             Location/Qualifiers
     source          1..3947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1438..1537)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1914..2705
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2798..3386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3287..3306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3540..3557
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3716..3743)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3835..3921)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgac cgaagaaaca cacccagacg atgacagcta tattgtgcgt gtcaaggctg
      241 tggttatgac cagagatgac tccagcgggg gatggttccc acaggaagga ggcgggatca
      301 gtcgcgtcgg ggtctgtaag gtcatgcacc ccgaaggcaa tggacgaagc ggctttctca
      361 tccatggtga acgacagaaa gacaaactgg tggtattgga atgctatgta agaaaggact
      421 tggtctacac caaagccaat ccaacgtttc atcactggaa ggtcgataat aggaagtttg
      481 gacttacttt ccaaagccct gctgatgccc gagcctttga caggggagta aggaaagcaa
      541 tcgaagacct tatagaaggt tcaacaacgt catcttccac catccataat gaagctgagc
      601 ttggcgatga tgacgttttt acaacagcta cagacagttc ttctaattcc tctcagaaga
      661 gagagcaacc tactcggaca atctcctctc ccacatcctg tgagcaccgg aggatttata
      721 ccctgggcca cctccacgac tcatacccca cagaccacta tcacctcgat cagccgatgc
      781 caaggcccta ccgccaggtg agcttcccgg acgacgacga ggagatcgtg cgcatcaacc
      841 cccgggagaa gatctggatg acggggtacg aggattaccg gcacgcaccc gtcaggggca
      901 agtacccgga cccctcggag gacgcggact cctcctacgt gcgcttcgcc aagggcgagg
      961 tccccaagca tgactacaac tacccctacg tggactcctc agactttggc ctaggcgagg
     1021 accccaaagg ccgcgggggc agcgtgatca agacgcagcc ctcccggggc aagtcgcggc
     1081 ggcggaagga ggacggagag cgctcgcggt gcgtgtactg cagggacatg ttcaaccacg
     1141 aggagaaccg ccggggccac tgccaggacg cgcccgactc cgtgagaact tgcatccgcc
     1201 gggtgagctg catgtggtgc gcggacagca tgctctatca ctgtatgtcg gaccccgagg
     1261 gagactatac agacccttgc tcgtgcgata ctagcgacga gaagttttgc ctccggtgga
     1321 tggctcttat tgccttgtct ttcctggccc cctgtatgtg ctgttacctg ccccttcggg
     1381 cctgctacca ctgcggagtg atgtgcaggt gctgtggcgg gaagcacaaa gcggccgcgt
     1441 caactttctt gtacaaagtt ggcattatag gaaagcattg cttatcaatt tgttgcaacg
     1501 aacaggtcac tatcagtcaa aataaaatca ttatttgcat gcaagcttag atcctctaga
     1561 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
     1621 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     1681 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1741 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     1801 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     1861 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
     1921 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     1981 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2041 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2101 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2161 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2221 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2281 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2341 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2401 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2461 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     2521 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     2581 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     2641 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     2701 aataaccctc gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2761 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2821 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2881 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2941 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3001 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3061 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3121 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3181 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3241 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3301 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3361 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3421 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3481 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3541 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3601 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3661 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3721 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3781 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3841 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3901 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcg
//
