LOCUS       sequence-126063-        3638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human SPRY2 [NM_005842.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3638)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E330 Hs.SPRY2-nostop
FEATURES             Location/Qualifiers
     source          1..3638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1129..1228)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1605..2396
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2489..3077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2978..2997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3231..3248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3407..3434)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3526..3612)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga ggccagagct cagagtggca acgggtcgca gcccttgctg cagacgcccc
      241 gtgacggtgg cagacagcgt ggggagcccg accccagaga cgccctcacc cagcaggtac
      301 atgtcttgtc tctggatcag atcagagcca tccgaaacac caatgagtac acagaggggc
      361 ctactgtcgt cccaagacct gggctcaagc ctgctcctcg cccctccact cagcacaaac
      421 acgagagact ccacggtctg cctgagcacc gccagcctcc taggctccag cactcgcagg
      481 tccattcttc tgcacgagcc cctctgtcca gatccataag cacggtcagc tcagggtcgc
      541 ggagcagtac gaggacaagt accagcagca gctcctctga acagagactg ctaggatcat
      601 ccttctcctc cgggcctgtt gctgatggca taatccgggt gcaacccaaa tctgagctca
      661 agccaggtga gcttaagcca ctgagcaagg aagatttggg cctgcacgcc tacaggtgtg
      721 aggactgtgg caagtgcaaa tgtaaggagt gcacctaccc aaggcctctg ccatcagact
      781 ggatctgcga caagcagtgc ctttgctcgg cccagaacgt gattgactat gggacttgtg
      841 tatgctgtgt gaaaggtctc ttctatcact gttctaatga tgatgaggac aactgtgctg
      901 acaacccatg ttcttgcagc cagtctcact gttgtacacg atggtcagcc atgggtgtca
      961 tgtccctctt tttgccttgt ttatggtgtt accttccagc caagggttgc cttaaattgt
     1021 gccaggggtg ttatgaccgg gttaacaggc ctggttgccg ctgtaaaaac tcaaacacag
     1081 tttgctgcaa agttcccact gtccccccta ggaactttga aaaaccaaca tcaactttct
     1141 tgtacaaagt tggcattata ggaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1201 ctatcagtca aaataaaatc attatttgca tgcaagctta gatcctctag accagccagg
     1261 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     1321 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1381 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1441 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     1501 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1561 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     1621 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     1681 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     1741 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     1801 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     1861 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     1921 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     1981 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2041 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2101 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2161 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2221 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2281 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2341 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     2401 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     2461 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2521 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2581 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     2641 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2701 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2761 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2821 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2881 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2941 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3001 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3061 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3121 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3181 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3241 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3301 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3361 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     3421 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     3481 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     3541 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     3601 tcgttttatt tgatgcctgg cagttcccta ctctcgcg
//
