LOCUS       sequence-126065-        3557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human SPRY3 [NM_005840.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3557)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E332 Hs.SPRY3-nostop
FEATURES             Location/Qualifiers
     source          1..3557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1048..1147)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1524..2315
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2408..2996
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2897..2916
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3150..3167
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3326..3353)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3445..3531)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga tgctgcggtg acagatgatt ttcaacaaat tctgcctatt gaacagctgc
      241 gctctactca tgctagcaat gactacgtgg aacggcctcc agccccctgt aaacaggccc
      301 tctccagccc ttcccttatt gtgcaaaccc acaagtctga ttggtctctg gctaccatgc
      361 ctacttctct cccccgcagt ctcagccagt gccatcaact gcagcccttg cctcagcatc
      421 tgagccaatc tagcattgcc agctcaatgt cccatagcac cactgcctct gatcaaaggc
      481 tcttggccag cattacaccc tcaccttcag gccaatccat catccgaacc caacctggag
      541 caggggtcca cccaaaggct gatggtgctc tgaagggaga agctgagcaa tctgcagggc
      601 accctagtga gcacctcttc atctgtgagg aatgtgggcg ctgcaagtgc gtcccctgca
      661 cagcagctcg ccctctcccc tcctgctggc tgtgcaacca gcgctgcctt tgctctgctg
      721 agagcctcct cgattatggc acttgtctct gctgtgtcaa gggcctcttc taccactgct
      781 ccactgatga tgaagacaac tgtgctgatg agccctgctc ttgtgggcct agttcttgct
      841 ttgtccgctg ggcagccatg agcctcatct ccctcttcct accctgcctg tgctgctacc
      901 tgcctacccg tggatgcctc catctgtgcc aacagggcta tgatagcctc cggcgaccag
      961 gctgccgctg caagaggcac accaacactg tgtgcagaaa gatctcttct ggtagtgcac
     1021 ccttccccaa ggcccaggaa aagtctgtat caactttctt gtacaaagtt ggcattatag
     1081 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1141 ttatttgcat gcaagcttag atcctctaga ccagccagga cagaaatgcc tcgacttcgc
     1201 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1261 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     1321 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1381 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     1441 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     1501 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     1561 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     1621 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     1681 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     1741 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     1801 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     1861 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     1921 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     1981 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     2041 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     2101 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     2161 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     2221 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     2281 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     2341 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2521 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     2581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2761 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     2821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3181 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3241 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     3301 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     3361 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     3421 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     3481 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     3541 agttccctac tctcgcg
//
