LOCUS       sequence-126068-        3995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human STK11 [NM_000455.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3995)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E335 Hs.STK11
FEATURES             Location/Qualifiers
     source          1..3995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1486..1585)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1962..2753
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2846..3434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3335..3354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3588..3605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3764..3791)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3883..3969)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga ggtggtggac ccgcagcagc tgggcatgtt cacggagggc gagctgatgt
      241 cggtgggtat ggacacgttc atccaccgca tcgactccac cgaggtcatc taccagccgc
      301 gccgcaagcg ggccaagctc atcggcaagt acctgatggg ggacctgctg ggggaaggct
      361 cttacggcaa ggtgaaggag gtgctggact cggagacgct gtgcaggagg gccgtcaaga
      421 tcctcaagaa gaagaagttg cgaaggatcc ccaacgggga ggccaacgtg aagaaggaaa
      481 ttcaactact gaggaggtta cggcacaaaa atgtcatcca gctggtggat gtgttataca
      541 acgaagagaa gcagaaaatg tatatggtga tggagtactg cgtgtgtggc atgcaggaaa
      601 tgctggacag cgtgccggag aagcgtttcc cagtgtgcca ggcccacggg tacttctgtc
      661 agctgattga cggcctggag tacctgcata gccagggcat tgtgcacaag gacatcaagc
      721 cggggaacct gctgctcacc accggtggca ccctcaaaat ctccgacctg ggcgtggccg
      781 aggcactgca cccgttcgcg gcggacgaca cctgccggac cagccagggc tccccggctt
      841 tccagccgcc cgagattgcc aacggcctgg acaccttctc cggcttcaag gtggacatct
      901 ggtcggctgg ggtcaccctc tacaacatca ccacgggtct gtaccccttc gaaggggaca
      961 acatctacaa gttgtttgag aacatcggga aggggagcta cgccatcccg ggcgactgtg
     1021 gccccccgct ctctgacctg ctgaaaggga tgcttgagta cgaaccggcc aagaggttct
     1081 ccatccggca gatccggcag cacagctggt tccggaagaa acatcctccg gctgaagcac
     1141 cagtgcccat cccaccgagc ccagacacca aggaccggtg gcgcagcatg actgtggtgc
     1201 cgtacttgga ggacctgcac ggcgcggacg aggacgagga cctcttcgac atcgaggatg
     1261 acatcatcta cactcaggac ttcacggtgc ccggacaggt cccagaagag gaggccagtc
     1321 acaatggaca gcgccggggc ctccccaagg ccgtgtgtat gaacggcaca gaggcggcgc
     1381 agctgagcac caaatccagg gcggagggcc gggcccccaa ccctgcccgc aaggcctgct
     1441 ccgccagcag caagatccgc cggctgtcgg cctgcaagca gcagtaatca actttcttgt
     1501 acaaagttgg cattatagga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     1561 tcagtcaaaa taaaatcatt atttgcatgc aagcttagat cctctagacc agccaggaca
     1621 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     1681 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     1741 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1801 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     1861 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1921 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     1981 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2041 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2101 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2161 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2221 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2281 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2341 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2401 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2461 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2521 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2581 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2641 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2701 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     2761 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2821 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2881 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2941 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3001 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3061 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3121 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3181 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     3241 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3301 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3361 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3421 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3481 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3541 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3601 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3661 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3721 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3781 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3841 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     3901 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     3961 ttttatttga tgcctggcag ttccctactc tcgcg
//
