LOCUS       sequence-126070-        4169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human STK3 [NM_006281.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4169)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E337 Hs.STK3
FEATURES             Location/Qualifiers
     source          1..4169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             839..847
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(1660..1759)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2136..2927
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3020..3608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3509..3528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3762..3779
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3938..3965)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4057..4143)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gcagccgccg gcgcctaaga gtaaactaaa aaagctgagt gaagacagtt
      241 tgactaagca gcctgaagaa gtttttgatg tattagagaa gcttggagaa gggtcttatg
      301 gaagtgtatt taaagcaata cacaaggaat ccggtcaagt tgtcgcaatt aaacaagtac
      361 ctgttgaatc agatcttcag gaaataatca aagaaatttc cataatgcag caatgtgaca
      421 gcccatatgt tgtaaagtac tatggcagtt attttaagaa tacagacctc tggattgtta
      481 tggagtactg tggcgctggc tctgtctcag acataattag attacgaaac aagacattaa
      541 tagaagatga aattgcaacc attcttaaat ctacattgaa aggactagaa tatttgcact
      601 ttatgagaaa aatacacaga gatataaaag ctggaaatat tctcctcaat acagaaggac
      661 atgcaaaatt ggcagatttt ggagtggctg gtcagttaac agatacaatg gcaaaacgca
      721 atactgtaat aggaactcca ttttggatgg ctcctgaggt gattcaagaa ataggctata
      781 actgtgtggc cgacatctgg tcccttggca ttacttctat agaaatggct gaaggaaaac
      841 ctccttatgc tgatatacat ccaatgaggg ctatttttat gattcccaca aatccaccac
      901 caacattcag aaagccagaa ctttggtccg atgatttcac cgattttgtt aaaaagtgtt
      961 tggtgaagaa tcctgagcag agagctactg caacacaact tttacagcat ccttttatca
     1021 agaatgccaa acctgtatca atattaagag acctgatcac agaagctatg gagatcaaag
     1081 ctaaaagaca tgaggaacag caacgagaat tggaagagga agaagaaaat tcggatgaag
     1141 atgagctgga ttcccacacc atggtgaaga ctagtgtgga gagtgtgggc accatgcggg
     1201 ccacaagcac gatgagtgaa ggggcccaga ccatgattga acataatagc acgatgttgg
     1261 aatccgactt ggggaccatg gtgataaaca gtgaggatga ggaagaagaa gatggaacta
     1321 tgaaaagaaa tgcaacctca ccacaagtac aaagaccatc tttcatggac tactttgata
     1381 agcaagactt caagaataag agtcacgaaa actgtaatca gaacatgcat gaacccttcc
     1441 ctatgtccaa aaacgttttt cctgataact ggaaagttcc tcaagatgga gactttgact
     1501 ttttgaaaaa tctaagttta gaagaactac agatgcggtt aaaagcactg gaccccatga
     1561 tggaacggga gatagaagaa cttcgtcaga gatacactgc gaaaagacag cccattctgg
     1621 atgcgatgga tgcaaagaaa agaaggcagc aaaactttta atcaactttc ttgtacaaag
     1681 ttggcattat aggaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1741 aaaataaaat cattatttgc atgcaagctt agatcctcta gaccagccag gacagaaatg
     1801 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     1861 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     1921 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1981 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     2041 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     2101 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     2161 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2221 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2281 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2341 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2401 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2461 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2521 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2581 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2641 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2701 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2761 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     2821 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2881 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     2941 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3001 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3061 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     3421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3661 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3721 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3781 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3841 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     3901 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     3961 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     4021 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4081 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4141 ttgatgcctg gcagttccct actctcgcg
//
