LOCUS       sequence-126071-        4166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human STK3 [NM_006281.3] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4166)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E338 Hs.STK3-nostop
FEATURES             Location/Qualifiers
     source          1..4166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             839..847
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(1657..1756)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2133..2924
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3017..3605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3506..3525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3759..3776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3935..3962)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4054..4140)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga gcagccgccg gcgcctaaga gtaaactaaa aaagctgagt gaagacagtt
      241 tgactaagca gcctgaagaa gtttttgatg tattagagaa gcttggagaa gggtcttatg
      301 gaagtgtatt taaagcaata cacaaggaat ccggtcaagt tgtcgcaatt aaacaagtac
      361 ctgttgaatc agatcttcag gaaataatca aagaaatttc cataatgcag caatgtgaca
      421 gcccatatgt tgtaaagtac tatggcagtt attttaagaa tacagacctc tggattgtta
      481 tggagtactg tggcgctggc tctgtctcag acataattag attacgaaac aagacattaa
      541 tagaagatga aattgcaacc attcttaaat ctacattgaa aggactagaa tatttgcact
      601 ttatgagaaa aatacacaga gatataaaag ctggaaatat tctcctcaat acagaaggac
      661 atgcaaaatt ggcagatttt ggagtggctg gtcagttaac agatacaatg gcaaaacgca
      721 atactgtaat aggaactcca ttttggatgg ctcctgaggt gattcaagaa ataggctata
      781 actgtgtggc cgacatctgg tcccttggca ttacttctat agaaatggct gaaggaaaac
      841 ctccttatgc tgatatacat ccaatgaggg ctatttttat gattcccaca aatccaccac
      901 caacattcag aaagccagaa ctttggtccg atgatttcac cgattttgtt aaaaagtgtt
      961 tggtgaagaa tcctgagcag agagctactg caacacaact tttacagcat ccttttatca
     1021 agaatgccaa acctgtatca atattaagag acctgatcac agaagctatg gagatcaaag
     1081 ctaaaagaca tgaggaacag caacgagaat tggaagagga agaagaaaat tcggatgaag
     1141 atgagctgga ttcccacacc atggtgaaga ctagtgtgga gagtgtgggc accatgcggg
     1201 ccacaagcac gatgagtgaa ggggcccaga ccatgattga acataatagc acgatgttgg
     1261 aatccgactt ggggaccatg gtgataaaca gtgaggatga ggaagaagaa gatggaacta
     1321 tgaaaagaaa tgcaacctca ccacaagtac aaagaccatc tttcatggac tactttgata
     1381 agcaagactt caagaataag agtcacgaaa actgtaatca gaacatgcat gaacccttcc
     1441 ctatgtccaa aaacgttttt cctgataact ggaaagttcc tcaagatgga gactttgact
     1501 ttttgaaaaa tctaagttta gaagaactac agatgcggtt aaaagcactg gaccccatga
     1561 tggaacggga gatagaagaa cttcgtcaga gatacactgc gaaaagacag cccattctgg
     1621 atgcgatgga tgcaaagaaa agaaggcagc aaaacttttc aactttcttg tacaaagttg
     1681 gcattatagg aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     1741 ataaaatcat tatttgcatg caagcttaga tcctctagac cagccaggac agaaatgcct
     1801 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     1861 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     1921 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     1981 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     2041 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2101 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     2161 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2221 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     2281 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2341 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2401 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2461 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2521 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2581 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2641 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2701 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2761 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     2821 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     2881 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     2941 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     3001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     3421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3841 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     3901 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3961 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     4021 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     4081 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     4141 atgcctggca gttccctact ctcgcg
//
