LOCUS       sequence-126074-        4883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human TBK1 [NM_013254.3] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4883)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E341 Hs.TBK1
FEATURES             Location/Qualifiers
     source          1..4883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2374..2473)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2850..3641
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3734..4322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4223..4242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4476..4493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4652..4679)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4771..4857)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgca gagcacttct aatcatctgt ggcttttatc tgatatttta ggccaaggag
      241 ctactgcaaa tgtctttcgt ggaagacata agaaaactgg tgatttattt gctatcaaag
      301 tatttaataa cataagcttc cttcgtccag tggatgttca aatgagagaa tttgaagtgt
      361 tgaaaaaact caatcacaaa aatattgtca aattatttgc tattgaagag gagacaacaa
      421 caagacataa agtacttatt atggaatttt gtccatgtgg gagtttatac actgttttag
      481 aagaaccttc taatgcctat ggactaccag aatctgaatt cttaattgtt ttgcgagatg
      541 tggtgggtgg aatgaatcat ctacgagaga atggtatagt gcaccgtgat atcaagccag
      601 gaaatatcat gcgtgttata ggggaagatg gacagtctgt gtacaaactc acagattttg
      661 gtgcagctag agaattagaa gatgatgagc agtttgtttc tctgtatggc acagaagaat
      721 atttgcaccc tgatatgtat gagagagcag tgctaagaaa agatcatcag aagaaatatg
      781 gagcaacagt tgatctttgg agcattgggg taacatttta ccatgcagct actggatcac
      841 tgccatttag accctttgaa gggcctcgta ggaataaaga agtgatgtat aaaataatta
      901 caggaaagcc ttctggtgca atatctggag tacagaaagc agaaaatgga ccaattgact
      961 ggagtggaga catgcctgtt tcttgcagtc tttctcgggg tcttcaggtt ctacttaccc
     1021 ctgttcttgc aaacatcctt gaagcagatc aggaaaagtg ttggggtttt gaccagtttt
     1081 ttgcagaaac tagtgatata cttcaccgaa tggtaattca tgttttttcg ctacaacaaa
     1141 tgacagctca taagatttat atacatagct ataatactgc tactatattt catgaactgg
     1201 tatataaaca aaccaaaatt atttcttcaa atcaagaact tatctacgaa gggcgacgct
     1261 tagtcttaga acctggaagg ctggcacaac atttccctaa aactactgag gaaaacccta
     1321 tatttgtagt aagccgggaa cctctgaata ccataggatt aatatatgaa aaaatttccc
     1381 tccctaaagt acatccacgt tatgatttag acggggatgc tagcatggct aaggcaataa
     1441 caggggttgt gtgttatgcc tgcagaattg ccagtacctt actgctttat caggaattaa
     1501 tgcgaaaggg gatacgatgg ctgattgaat taattaaaga tgattacaat gaaactgttc
     1561 acaaaaagac agaagttgtg atcacattgg atttctgtat cagaaacatt gaaaaaactg
     1621 tgaaagtata tgaaaagttg atgaagatca acctggaagc ggcagagtta ggtgaaattt
     1681 cagacataca caccaaattg ttgagacttt ccagttctca gggaacaata gaaaccagtc
     1741 ttcaggatat cgacagcaga ttatctccag gtggatcact ggcagacgca tgggcacatc
     1801 aagaaggcac tcatccgaaa gacagaaatg tagaaaaact acaagtcctg ttaaattgca
     1861 tgacagagat ttactatcag ttcaaaaaag acaaagcaga acgtagatta gcttataatg
     1921 aagaacaaat ccacaaattt gataagcaaa aactgtatta ccatgccaca aaagctatga
     1981 cgcactttac agatgaatgt gttaaaaagt atgaggcatt tttgaataag tcagaagaat
     2041 ggataagaaa gatgcttcat cttaggaaac agttattatc gctgactaat cagtgttttg
     2101 atattgaaga agaagtatca aaatatcaag aatatactaa tgagttacaa gaaactctgc
     2161 ctcagaaaat gtttacagct tccagtggaa tcaaacatac catgacccca atttatccaa
     2221 gttctaacac attagtagaa atgactcttg gtatgaagaa attaaaggaa gagatggaag
     2281 gggtggttaa agaacttgct gaaaataacc acattttaga aaggtttggc tctttaacca
     2341 tggatggtgg ccttcgcaac gttgactgtc tttaatcaac tttcttgtac aaagttggca
     2401 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     2461 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     2521 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     2581 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     2641 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     2701 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2761 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2821 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     2881 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2941 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     3001 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     3061 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     3121 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     3181 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     3241 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     3301 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     3361 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     3421 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     3481 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     3541 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     3601 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     3661 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     3721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3841 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     4141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4381 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4441 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4501 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4561 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     4621 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     4681 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     4741 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     4801 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     4861 cctggcagtt ccctactctc gcg
//
