LOCUS       sequence-126092-        4208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human YAP1 [NM_001130145.2] with stop codon 
            (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4208)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E359 Hs.YAP1
FEATURES             Location/Qualifiers
     source          1..4208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1699..1798)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2175..2966
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3059..3647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3548..3567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3801..3818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3977..4004)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4096..4182)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgga tcccgggcag cagccgccgc ctcaaccggc cccccagggc caagggcagc
      241 cgccttcgca gcccccgcag gggcagggcc cgccgtccgg acccgggcaa ccggcacccg
      301 cggcgaccca ggcggcgccg caggcacccc ccgccgggca tcagatcgtg cacgtccgcg
      361 gggactcgga gaccgacctg gaggcgctct tcaacgccgt catgaacccc aagacggcca
      421 acgtgcccca gaccgtgccc atgaggctcc ggaagctgcc cgactccttc ttcaagccgc
      481 cggagcccaa atcccactcc cgacaggcca gtactgatgc aggcactgca ggagccctga
      541 ctccacagca tgttcgagct cattcctctc cagcttctct gcagttggga gctgtttctc
      601 ctgggacact gacccccact ggagtagtct ctggcccagc agctacaccc acagctcagc
      661 atcttcgaca gtcttctttt gagatacctg atgatgtacc tctgccagca ggttgggaga
      721 tggcaaagac atcttctggt cagagatact tcttaaatca catcgatcag acaacaacat
      781 ggcaggaccc caggaaggcc atgctgtccc agatgaacgt cacagccccc accagtccac
      841 cagtgcagca gaatatgatg aactcggctt caggtcctct tcctgatgga tgggaacaag
      901 ccatgactca ggatggagaa atttactata taaaccataa gaacaagacc acctcttggc
      961 tagacccaag gcttgaccct cgttttgcca tgaaccagag aatcagtcag agtgctccag
     1021 tgaaacagcc accacccctg gctccccaga gcccacaggg aggcgtcatg ggtggcagca
     1081 actccaacca gcagcaacag atgcgactgc agcaactgca gatggagaag gagaggctgc
     1141 ggctgaaaca gcaagaactg cttcggcagg caatgcggaa tatcaatccc agcacagcaa
     1201 attctccaaa atgtcaggag ttagccctgc gtagccagtt accaacactg gagcaggatg
     1261 gtgggactca aaatccagtg tcttctcccg ggatgtctca ggaattgaga acaatgacga
     1321 ccaatagctc agatcctttc cttaacagtg gcacctatca ctctcgagat gagagtacag
     1381 acagtggact aagcatgagc agctacagtg tccctcgaac cccagatgac ttcctgaaca
     1441 gtgtggatga gatggataca ggtgatacta tcaaccaaag caccctgccc tcacagcaga
     1501 accgtttccc agactacctt gaagccattc ctgggacaaa tgtggacctt ggaacactgg
     1561 aaggagatgg aatgaacata gaaggagagg agctgatgcc aagtctgcag gaagctttga
     1621 gttctgacat ccttaatgac atggagtctg ttttggctgc caccaagcta gataaagaaa
     1681 gctttcttac atggttataa tcaactttct tgtacaaagt tggcattata ggaaagcatt
     1741 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgca
     1801 tgcaagctta gatcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1861 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     1921 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     1981 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2041 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2101 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2161 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2221 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2281 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2341 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2401 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2461 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2521 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2581 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2641 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2701 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2761 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2821 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2881 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     2941 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3001 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3181 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3421 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     3481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3721 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3781 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3841 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3901 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3961 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4021 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4081 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4141 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4201 ctctcgcg
//
