LOCUS       sequence-126102-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An endoplasmic reticulum (ER) marker used to visulaize the ER 
            structure in Drosophilia S2 cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Okajima T, Xu A, Lei L, Irvine KD
  TITLE     Chaperone activity of protein O-fucosyltransferase 1 promotes notch 
            receptor folding.
  JOURNAL   Science. 2005 Mar 11;307(5715):1599-603. Epub 2005 Feb 3.
  PUBMED    15692013
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-03-11"; volume: "307(5715)"; pages: "1599-603. Epub 2005
            Feb 3"
COMMENT     Sequence Label: pMT-Bip-GFP:V5:KDEL
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     3..53
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="KLCILLAVVAFVGLSLG"
     primer_bind     complement(120..148)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     743..763
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             837..878
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(928..945)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(1002..1021)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgaagttatg catattactg gccgtcgtgg cctttgttgg cctctcgctc gggagatctc
       61 catggcccgg ggtaccggta gaaaaaatgg ctagcaaagg agaagaactt ttcactggag
      121 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      181 gagagggtga aggtgatgct acatacggaa agcttaccct taaatttatt tgcactactg
      241 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct
      301 tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
      361 gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg
      421 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta
      481 aagaagatgg aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat
      541 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgtcacaaca
      601 ttgaagatgg atccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      661 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc
      721 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
      781 atggcatgga tgatccagca cagtggcggc cgctcgagtc tagagggccc ttcgaaggta
      841 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtagc gagaaggacg
      901 agctgtgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct
      961 gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca
     1021 caactagaat gcagtgaaaa a
//
