LOCUS       sequence-126103-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent
            protein-based calcium integrator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Fosque BF, Sun Y, Dana H, Yang CT, Ohyama T, Tadross MR, Patel R, 
            Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER
  TITLE     Neural circuits. Labeling of active neural circuits in vivo with 
            designed calcium integrators.
  JOURNAL   Science. 2015 Feb 13;347(6223):755-60. doi: 10.1126/science.1260922.
  PUBMED    25678659
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-02-13- 13"; volume: "347"; 
            issue: "6223"; pages: "755-60"
COMMENT     Sequence Label: pcDNA3-CaMPARI
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..85
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        105..123
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     105..122
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(154..210)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
       61 tggcaactag aaggcacagt cgaggctgat cagcgagctc tagcatttag gtgacactat
      121 agaatagggc cctctagatg catgctcgag ttatgagctc agccgaccta tagctctgac
      181 tgcgtgacct gtcttattcc acttacgacg tgatgaacgc gtccagctcg gcatccattt
      241 cagcgtcttc ttctgcataa ctggaccatt ggcgggaaag ttggtaccat aaaatcgaac
      301 tttattatag aaagtgtccc cttccattgt tatgtcgttt ctggcatagc aaatgccccc
      361 gtcttcgaaa gtcatgcttc gttcccacga atacccctta ggaaacgact gcttaaaata
      421 gtcttgtatg ttgtctggat atttcacgaa taccctgttg ccgtaatgga atgcagtggt
      481 caggatatca aaggcaaaag gcagaggtcc gccctctttg acttcaagat ccatggtctg
      541 ttttccctca taaggcttgc ctgtaccatc tccgtcgatc acaaagtggt gcccgtttac
      601 gttgccttcc atacggagtt tgatcttcat gtctggctta atcgcactca ccatggatcc
      661 gccggtaccg cctcgtctgg cattgtcagg caatccagaa tgagcaacag catactcata
      721 cagcttaacc ttgttgtaat ctttgtcatg gcttaaaatc tcaatgcagt ggtccacaaa
      781 gtgcacgcct ggtaacttga cacccttctc cttagctttg taagtagttc tgaagtcaca
      841 tcggtaatgg gcatttcctt caagcaacaa gatcatatga atatcacccg tcagcactcc
      901 atcacgcaca tagattttct cgcactcgag cttcgctgtc atcatttgta caaactcttc
      961 gtagtttacc tgaccatccc catcgatgtc tgcttccctg atcatttcat caacctcttc
     1021 atctgttaac ttctctccaa ggtttgtcat cacgtggcga agctctgctg cactgatgta
     1081 gccattgcca tccttatcaa acacacggaa cgcttctcta att
//
