LOCUS       sequence-126104-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic fragment of Drosophila aubergine was cloned from w1 
            background and inserted into pattB plasmid; mutagenesis PCR was used
            to change the catalytic residue DDH to ADH to express cat-dead Aub.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  TITLE     pattB-aubergine-WT-gf
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pattB-aubergine-ADH-gf
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    145..178
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt
       61 ttatatatat caaaaataac aactataata ataagaatac atttaattta gaaaatgctt
      121 ggatttcact ggaactaggc tagcataact tcgtataatg tatgctatac gaagttatgc
      181 tagcggatcc gggaattggg aattcgttaa cagatctgcg gccgctgcat aaggttgccc
      241 taccaattgc taatactata aaataacaaa tatcaaaaca atctgttttc acattaagca
      301 tttaaagaat cgctgtctag attcaactta atccatcaaa tttagtatat tatataatag
      361 atatttctta aaaccataat taactttata tcgaagggta agtacatgtt aaaaagtaca
      421 aaattaatta atctgattaa aacgagttgt tttcgtaaat ttcttctatt tttgtattgg
      481 acagtactta catgtaaata aacaagttga taaacttgct ataagtataa tttcttgatt
      541 gaagaatgta ttttaattta gtgtgtaaac gtagtatata attgtcaaat agagaaaaat
      601 caatacgata ttattttgta tttaatatac tttataatac tttaacataa gttatacatg
      661 ggactataaa gtgatataca tatgtatcca ttgtaaaatc ttaggtgcac acttaatttt
      721 attacgactg ttttaaacct atata
//
