LOCUS       sequence-126107-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An endoplasmic reticulum (ER) marker used to visulaize the ER 
            structure in Drosophilia S2 cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Okajima T, Xu A, Lei L, Irvine KD
  TITLE     Chaperone activity of protein O-fucosyltransferase 1 promotes notch 
            receptor folding.
  JOURNAL   Science. 2005 Mar 11;307(5715):1599-603. Epub 2005 Feb 3.
  PUBMED    15692013
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-03-11"; volume: "307(5715)"; pages: "1599-603. Epub 2005
            Feb 3"
COMMENT     Sequence Label: pMT-Bip-GFP:V5:KDEL
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..59
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(109..150)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(224..244)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     839..867
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     sig_peptide     complement(934..987)
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
ORIGIN
        1 gtctggatcc tcgatcgagg ctgatcagcg agctcaggcc ttagaaggca cagtcgaggc
       61 tgatcagcgg gtttaaactc acagctcgtc cttctcgcta ccggtacgcg tagaatcgag
      121 accgaggaga gggttaggga taggcttacc ttcgaagggc cctctagact cgagcggccg
      181 ccactgtgct ggatcatcca tgccatgtgt aatcccagca gcagttacaa actcaagaag
      241 gaccatgtgg tcacgctttt cgttgggatc tttcgaaagg gcagattgtg tcgacaggta
      301 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      361 tgctagttga acggatccat cttcaatgtt gtgacgaatt ttgaagttag ctttgattcc
      421 attcttttgt ttgtctgccg tgatgtatac attgtgtgag ttatagttgt actcgagttt
      481 gtgtccgaga atgtttccat cttctttaaa atcaatacct tttaactcga tacgattaac
      541 aagggtatca ccttcaaact tgacttcagc acgcgtcttg tagttcccgt catctttgaa
      601 agatatagtg cgttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
      661 tttcatatga tccggataac gggaaaagca ttgaacacca taagagaaag tagtgacaag
      721 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagctttcc
      781 gtatgtagca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc
      841 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat
      901 tttttctacc ggtaccccgg gccatggaga tctcccgagc gagaggccaa caaaggccac
      961 gacggccagt aatatgcata acttcatatt gagatcggat ccccccttta gttgcactga
     1021 gatgattcac ttgattttgc tgctgaccac aactgatgca cattggccag atgctctcgg
     1081 ttt
//
