LOCUS       sequence-126112-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual-promoter plasmid to express knockdown-proof human TACC3 
            (682-688aa ch-TOG binding site deleted) tagged with EGFP along with 
            shRNA to knock down endogenous TACC3 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
  TITLE     TACC3-ch-TOG track the growing tips of microtubules independently of
            clathrin and Aurora-A phosphorylation.
  JOURNAL   Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
  PUBMED    25596274
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/bio"; 
            journalName: "Biol Open"; date: "2015-01-16- 16"; volume: "4"; 
            issue: "2"; pages: "170-9"
COMMENT     Sequence Label: pBrain-GFP-TACC3KDP∆682-688-shTACC3
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcttggagat gaggtcgtcg cagatcctgg tcagctcctc gttctcttta gtcttctgct
       61 ccactgtctt ctccagcgac tggatgcgca tctgctcctt cctcaggctg gcctggaggg
      121 ccaacgcttc cgcctgggcc ttgctccgga cctgggcgat ctcctcgttt gccagctgca
      181 gcttctcctc cgcgtgggcc ttcagggctt ggtacctctg gccctcctgg gtgatccttg
      241 ccaggtaatc ctccacgcac ttcttcagtg actcttcgtt cttgcggtag ccctcgatca
      301 cctctttctg tttctcaaaa cgcttgaaga ggtcggagaa ggacttctcc atggagttca
      361 gatctgtggt aagttggtct ttttctttta gaactttctg gatttcagct ttggaaagtt
      421 ccttctgctt ctgaacttcc tcttcgaacc tgtccatgat cttccccagt tccaggttct
      481 tcccgtggag ctcctcacac ctgctcctca gctcccggtt ctcctcctgt gtcgccttta
      541 ccactgcatc caggtccttc tggctgtact ggagcaggtc cactataggt ccggtggaca
      601 ggggtgggcc cccaggcgcg ggaacacctg ggggtgggcc tggcacagga atgtccagtg
      661 ctcccaagaa atcgaactcc acaagcttgg cttcccgcgg acgtcctgag ggagtctcat
      721 ttgcaccgtg catgctgctg gtctcggtgg ccacgggcac tggtctacca ggactgtccc
      781 tcaggaggg
//
