LOCUS       sequence-126116-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of 2GFP tag at Atg9 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD209
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..769)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(56..769)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ataataaaaa tcataaatca taagaaattc gcttatttag aagtggcgcg ccttatttat
       61 ataattcatc cataccatgg gtaataccag cagcagtaac aaattctaac aagaccatgt
      121 ggtctctctt ttcgtttgga tctttggata aggcagattg agtggataag taatggttgt
      181 ctggtaacaa gactggacca tcaccaattg gagtattttg ttgataatgg tcagctaatt
      241 gaacagaacc atcttcaatg ttgtgtctaa ttttgaagtt aactttgata ccattctttt
      301 gtttgtcagc catgatgtaa acattgtgag agttatagtt gtattccaat ttgtgaccta
      361 aaatgttacc atcttcttta aaatcaatac cttttaattc gattctatta actaaggtat
      421 caccttcaaa cttgacttca gctctggtct tgtagttacc gtcatctttg aaaaaaatag
      481 ttctttcttg aacataacct tctggcatgg cagacttgaa aaagtcatgt tgtttcatat
      541 gatctgggta tctagaaaaa cattgaacac cataagttaa agtagtgact aaggttggcc
      601 atggaactgg caatttacca gtagtacaaa taaattttaa ggtcaattta ccgtaagtag
      661 catcaccttc accttcaccg gagacagaaa atttgtgacc attaacatca ccatctaatt
      721 caaccaaaat tgggacaaca ccagtgaata attcttcacc tttagacatg aatggcttct
      781 ctagatcacg cgttggaact ggaattttgt acaattcatc aataccatgg gtaataccag
      841 cagcagtaac aaattctaac aagaccatgt ggtctctctt tt
//
