LOCUS       sequence-126127-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of GFP tag at Atg6 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD106
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     53..69
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     53..69
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac
       61 tagtggatcc cctttgccac tataaatgga ttgagattag gcagtatacc agaatcagtg
      121 gtaccttgga aggaaatcaa tgcagcactg ggccaattga ttcttttact ggctaccata
      181 aacaaaaacc tgaagataaa tttagtagac tacgaattac aaccgatggg ttcattttcc
      241 aaaatcaaga aaagaatggt taacagtgtc gaatataata attccactac gaacgctccc
      301 ggtgattggt tgattctgcc tgtctactat gatgagaatt tcaatttggg gagaatcttc
      361 cgcaaggaaa cgaagtttga taaatcatta gaaacaacat tagaaataat aagcgaaatc
      421 actcggcagc tttctacgat agcgtcatca tattcatctc aaacactcac aacaagccaa
      481 gatgaatctt caatgaataa cgcaaatgac gttgaaaata gtacttccat tctcgagcta
      541 ccctatatca tgaataagga caagattaat ggattatccg tcaaattgca tggtagcagt
      601 cccaaccttg aatggacaac ggctatgaaa tttttattaa ccaatgtaaa gtggttgtta
      661 gcgttttctt ccaatttact atccaagtca attaccttaa gccctacagt gaattacaat
      721 gataagacca tcagcggaaa cgcttcgtac gctgcaggtc gacggatcgg tgacggtgct
      781 ggtttaatta acatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt
      841 gaattagatg gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat
      901 gctacttacg gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca
      961 tggccaacct tagtcactac tttaacttat ggtgttcaat gtttttctag atacccagat
     1021 catatgaaac aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga
     1081 actatttt
//
