LOCUS       sequence-126130-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration of GFP tag at Atg14 C terminus, use auxotrophic marker 
            URA3(Candida albicans).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Li D, Song JZ, Shan MH, Li SP, Liu W, Li H, Zhu J, Wang Y, Lin J, 
            Xie Z
  TITLE     A fluorescent tool set for yeast Atg proteins.
  JOURNAL   Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
  PUBMED    25998947
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(6):954-60. doi"; pages: " 
            10.1080/15548627.2015.1040971"
COMMENT     Sequence Label: LD114
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     185..207
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     199..216
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     200..216
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     223..242
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        223..241
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
       61 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      121 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      181 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
      241 ggcgaattgg agctccaccg cggaacgatt ccatcaacat catgtggaag tatattagct
      301 tcttctcaga cattcttatg attaaacttc cctacacaaa taaaatctgt gaacaaccca
      361 tgtttgagtt ttccgatagc atacagacag tagtacaaag gttgatcaag cttatcataa
      421 atattttaca gatatgtaga catttgaaac tcgtaccttc aacacccatg gatatcccat
      481 ggctactgga ccagtacgat gtggatgggc tgttctataa tatggtgaaa cggaacaaga
      541 tgaagtgtag gtccgtctcg ctatattgga cttttgggat gttgtactcg atggttttgg
      601 ataacatgaa taatccacaa agaggacatc ctgcaaggcg gaccgcacca cctccaacag
      661 tcacaggacc tcatgaccga tggtacgtgg taggcgcttc gtacgctgca ggtcgacgga
      721 tcggtgacgg tgctggttta attaacatgt ctaaaggtga agaattattc actggtgttg
      781 tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct gtctccggtg
      841 aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt actactggta
      901 aattgccagt tccatggcca accttagtca ctactttaac ttatggtgtt caatgttt
//
