LOCUS       sequence-126135-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM585
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    116..132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 acaggaacac ttaacggctg acatgggaat tcccctccac cgcggtggta caaagaaaat
       61 tcgacaaact gttatttttc tatctattta aattgtgagc ggataacaat ttgaattgtg
      121 agcggataac aattaccttt gtcggcaaat aaagatattc tcgtcaaaca aatataaata
      181 atataaacat ggtttttact ctggaagatt ttgttggcga ttggcgtcag accgcgggtt
      241 ataatttgga tcaagtcctg gaacagggtg gcgtaagctc tctgttccag aacctgggtg
      301 tgagcgtgac gccgattcag cgcatcgttc tgtccggcga gaacggtctg aaaattgata
      361 ttcatgtgat catcccgtac gaaggcctga gcggtgacca aatgggtcaa atcgagaaaa
      421 tctttaaagt cgtctaccca gttgacgatc accacttcaa ggttatcttg cattacggta
      481 cgctggtgat tgatggtgtg accccgaata tgattgacta tttcggccgt ccgtatgaag
      541 gcattgccgt ttttgacggt aaaaagatca ccgtcaccgg taccctgtgg aatggcaata
      601 agattattga cgagcgtctg attaacccgg acggcagcct gctgttccgc gtgaccatca
      661 acggtgtcac gggttggcgt ctgtgcgagc gcatcctggc ataatgaact gcacttgctt
      721 tgataattaa tgataaacaa tctaaaagca ctctaatcgt tatcggagtg cttttagatt
      781 actaatcaaa ttgcttctac taattgccta tcttccagtg atggaacagc att
//
