LOCUS       sequence-126139-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM596
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    103..119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    159..175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac cgcggtggta
       61 caaagaaaat tcgacaaact gttatttttc tatctattta aattgtgagc ggataacaat
      121 ttgggtggga aactttagtt atgtaccttt gtcggcaatt gtgagcggat aacaattaaa
      181 taaagatatt ctcgtcaaac aaatataaat aatataaaca tggtttttac tctggaagat
      241 tttgttggcg attggcgtca gaccgcgggt tataatttgg atcaagtcct ggaacagggt
      301 ggcgtaagct ctctgttcca gaacctgggt gtgagcgtga cgccgattca gcgcatcatt
      361 ctgtccggcg agaacggtct gaaaattgat attcatgtga tcatcccgta cgaaggcctg
      421 agcggtgacc aaatgggtca aatcgagaaa atctttaaag tcgtctaccc agttgacgat
      481 caccacttca aggttatctt gcattacggt acgctggtga ttgatggtgt gaccccgaat
      541 atgattgact atttcggccg tccgtatgaa ggcattgccg tttttgacgg taaaaagatc
      601 accgtcaccg gtaccctgtg gaatggcaat aagattattg acgagcgtct gattaacccg
      661 gacggcagcc tgctgttccg cgtgaccatc aacggtgtca cgggttggcg tctgtgcgag
      721 cgcatcctgg cataatgaac tgcacttgct ttgataatta atgataaaca atctaaaagc
      781 actctaatcg ttatcggagt gcttttagat tactaatcaa attgcttcta ctaattgcct
      841 atcttccagt gatggaacag catt
//
