LOCUS       sequence-126149-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Medium P2 activator plasmid for two-strain system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pC220
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..744)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 ctgcagggcg taattttcgt cgttcgctgc tttgtacagt tcatccatac catgcgtgat
       61 gcccgctgcg gttacgaact ccagcagaac catatgatcg cgtttctcgt tcggatcttt
      121 agacagaacg ctttgcgtgc tcagatagtg attgtctggc agcagaacag gaccatcacc
      181 gattggagtg ttttgctggt agtgatcagc cagctgcacg ctgccatcct ccacgttgtg
      241 gcgaatttta aaattcgctt taatgccatt tttttgttta tcggcggtga tgtaaacatt
      301 gtcgctgatg taattgtatt ccagcttatg gcccaggata ttgccgtctt ctttaaagtc
      361 aatgcctttc agctcaatgc ggtttaccag ggtatcgcct tcaaatttca cttccgcacg
      421 cgttttgtac gtgccgtcat ccttaaagga aatcgtgcgt tcctgcacat agccttccgg
      481 catggcggac ttgaagaagt catgctgctt catatggtcc ggataacgag caaagcactg
      541 aacaccccaa gtcagcgtcg ttaccagagt cggccaaggt accggcagtt taccagtagt
      601 acagatgaac ttcagcgtca gtttaccatt agttgcgtca ccttcaccct cgccacgcac
      661 ggaaaactta tgaccgttga catcaccatc cagttccacc agaataggga cgacaccagt
      721 gaacagctct tcgcctttac gcatactagt a
//
