LOCUS       sequence-12615-e        5527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Kaykas A, Worringer K, Sugden B
  TITLE     CD40 and LMP-1 both signal from lipid rafts but LMP-1 assembles a 
            distinct, more efficient signaling complex.
  JOURNAL   EMBO J. 2001 Jun 1. 20(11):2641-54.
  PUBMED    11387199
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2001 Jun 1. 20(11):2641-54."
COMMENT     Sequence Label: p1990 SV40-LMP1
FEATURES             Location/Qualifiers
     source          1..5527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9..200
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(32..52)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          395..967
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     975..994
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1022..1041
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2118..2127
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2523..2644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2560..2579)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2614..2633
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2678..2695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2849..3437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2929..2948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3608..4399,4400..4468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4231..4250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4469..4573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4641..4659
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4698..4720)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4820..4839
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4906..5361)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5043..5064)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5255..5274
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5491..5513
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5505..5522
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5506..5522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      421 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      481 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
      541 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
      601 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
      661 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
      721 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
      781 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
      841 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
      901 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
      961 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1021 gtaatacgac tcactatagg gcgaattccc ggggatccgt tgaggatgga acacgacctt
     1081 gagaggggcc caccgggccc gcgacggccc cctcgaggac cccccctctc ctcttcccta
     1141 ggccttgctc tccttctcct cctcttggcg ctactgtttt ggctgtacat cgttatgagt
     1201 gactggactg gaggagccct ccttgtcctc tattcctttg ctctcatgct tataattata
     1261 attttgatca tctttatctt cagaagagac cttctctgtc cacttggagc cctttgtata
     1321 ctcctactga tgatcaccct cctgctcatc gctctctgga atttgcacgg acaggcattg
     1381 taccttggaa ttgtgctgtt catcttcggg tgcttacttg tcttaggtct ctggatctac
     1441 ttattggaga ttctctggcg acttggtgcc accatctggc agcttttggc cttcttccta
     1501 gccttcttcc tagacctcat cctgctcatt attgctctct atctacaaca aaactggtgg
     1561 actctattgg ttgatctcct ttggctcctc ctgtttctgg cgattttaat ctggatgtat
     1621 taccatggac aacgacacag tgatgaacac caccacgatg actccctccc gcaccctcaa
     1681 caagctaccg atgattctgg ccatgaatct gactctaact ccaacgaggg cagacaccac
     1741 ctgctcgtga gtggagccgg cgacggaccc ccactctgct ctcaaaacct aggcgcacct
     1801 ggaggtggtc ctgacaatgg cccacaggac cctgacaaca ctgatgacaa tggcccacag
     1861 gaccctgaca acactgatga caatggccca catgacccgc tgcctcagga ccctgacaac
     1921 actgatgaca atggcccaca ggaccctgac aacactgatg acaatggccc acatgacccg
     1981 ctgcctcata gccctagcga ctctgctgga aatgatggag gccctccaca attgacggaa
     2041 gaggttgaaa acaaaggagg tgaccagggc ccgcctttga tgacagacgg aggcggcggt
     2101 catagtcatg attccggcca tggcggcggt gatccacacc ttcctacgct gcttttgggt
     2161 tcttctggtt ccggtggaga tgatgacgac ccccacggcc cagttcagct aagctactat
     2221 gactaacctt tctttacttc taggcattac catgtcatag gcttgcctga ctgactctcc
     2281 ctccatttac tgggaatgcc ttagctaatc accttaactg gcacacactc ccttagccac
     2341 actgtctgtc taggctgaaa agccacattc atattctatt tcaaaacaag gggaaaggag
     2401 gacatgcgag aattggcaga cacctttacc cagcccttaa cacaccacac aggtagcaag
     2461 gacccgggtc tggccactcg agacgcgtca gatctagctg actaatgatc ttattaaagc
     2521 agaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2581 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2641 cttatcatgt ctggtcgact ctagactctt ccgcttcctc gctcactgac tcgctgcgct
     2701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     3061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3601 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3661 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3721 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3781 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3841 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3901 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3961 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4021 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4081 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4141 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4201 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4261 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4321 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4381 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4441 gggcgacacg gaaatgttga atactcatac tcttcttttt tcaatattat tgaagcattt
     4501 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4561 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     4621 tcatgacatt aacctataaa aataggcgta tcacgaggcc cctttcgtct cgcgcgtttc
     4681 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     4741 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4801 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     4861 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta
     4921 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4981 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     5041 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     5101 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     5161 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     5221 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     5281 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     5341 atgcgccgct acagggcgcg tcgcgccatt cgccattcag gctacgcaac tgttgggaag
     5401 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaagggggga tgtgctgcaa
     5461 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     5521 gtgaatt
//
