LOCUS       sequence-126161-       10859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, 
            AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM705
FEATURES             Location/Qualifiers
     source          1..10859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(28..54,55..57,58..2544,2545..2547,2548..4134)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        4294..4369
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4815..5897
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4834..4853)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(9060..9128,9129..9920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9278..9297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            10051..10160
                     /note="incP origin of transfer"
     rep_origin      10331..10719
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    10793..10809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    10826..10842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgtcaaacaa atataaataa tataaacatg gataagaaat actcaatagg cttagctatc
       61 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
      121 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
      181 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg
      241 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
      301 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
      361 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
      421 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg
      481 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
      541 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
      601 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
      661 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
      721 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
      781 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
      841 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg
      901 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa
      961 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac
     1021 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt
     1081 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa
     1141 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg
     1201 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc
     1261 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca
     1321 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat tccttattat
     1381 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa
     1441 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt
     1501 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat
     1561 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact
     1621 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta
     1681 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa
     1741 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta
     1801 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa
     1861 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg
     1921 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt
     1981 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg
     2041 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc
     2101 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca
     2161 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct
     2221 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg
     2281 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa
     2341 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta
     2401 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc
     2461 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat
     2521 cgtttaagtg attatgatgt cgatgccatt gttccacaaa gtttccttaa agacgattca
     2581 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca
     2641 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta
     2701 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt
     2761 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg
     2821 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga
     2881 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa
     2941 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc
     3001 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt
     3061 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa
     3121 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca
     3181 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa
     3241 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa
     3301 gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga gtcaatttta
     3361 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat
     3421 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa
     3481 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tatggaaaga
     3541 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa
     3601 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa
     3661 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa
     3721 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat
     3781 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag
     3841 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt
     3901 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat
     3961 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt
     4021 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc
     4081 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga ctgaattaat
     4141 gcggctgcaa tttttttggg cggggccgcc caaaaaaatc ctagcaccct gcagcagtac
     4201 tgcttgacca taagaacaaa aaaacttccg ataaagtttg gaagataaag ctaaaagttc
     4261 ttatctttgc agtgaaaatg gggtgtatcc tgcgttttag agctagaaat agcaagttaa
     4321 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctag
     4381 atgatgatta aggatcttgc gtacaatttg acgatcgttt cacgctaaga tgctggctaa
     4441 ttgcctatct tccagtgatg gaacagcatt tgtgcattgg ctgcaacaat cagccttgat
     4501 ctggaagaag caatgaaagc tgctgttaag tctccgaatc aggtattgtt cctgacaggt
     4561 gtattcccat ccggtaaacg cggatacttt gcagttgatc tgactcagga ataaattata
     4621 aattaaggta agaagattgt aggataagct aatgaaatag aaaaaggatg ccgtcacaca
     4681 acttgtcggc attctttttt gttttattag ttgaaaatat agtgaaaaag ttgcctaaat
     4741 atgtatgtta acaaattatt tgtcgtaact ttgcactcca aatctgtttt taacatatgg
     4801 cactagtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
     4861 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
     4921 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     4981 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     5041 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     5101 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
     5161 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     5221 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     5281 tcaacagtat tattttctcc catgaggacg gtacgcgact gggcgtggag catctggtcg
     5341 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     5401 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     5461 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     5521 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     5581 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
     5641 ccgaggacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
     5701 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     5761 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     5821 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5881 tggaaagcgg gcagtgagct ttcctcggta ccaaattcca gaaaagaggc ctcccgaaag
     5941 gggggccttt tttcgttttg gtcctacttg tgcctgttct atttccgaac cgaccgcttg
     6001 tatgaatcca tcaaaattcg ttttctctat gttggattcc ttgttgctca tattgtgatg
     6061 ataatttcta caaatatagt cattggtaac tatctatgaa actgtttgat acttttatag
     6121 ttgattaaac ttgttcatgg catttgcctt aatatcatcc gctatgtcaa tgtagggttt
     6181 catagctttg tagtcgctgt gtcccgtcca tttcatgacc acctgtgccg ggattccgag
     6241 agccagcgca ttgcagatga atgtccttct tcctgcatgg gtactgagca aagcgtattt
     6301 gggtgtgact tcatcaatac gttcatttcc cttgtagtag gtttcccgta caggctcgtt
     6361 gatttctgcc agttcgccca gctctttcag gtaatcgttc atcttctggt tgctgatgac
     6421 gggcagagcc atgtaattct cgaaatggat gtccttgtat ttgtccagta tggctttgct
     6481 gtatttgttc agttcaatcg tcaggctgtc ggcagtcttg actgtggtta tttcgatgtg
     6541 gtcggacttc acatcgcttc ttttcagatt gcgaacatcc gaataccgca aactcgtaaa
     6601 gcagcagaac aggaaaacat cacgcacacg ttccaggtat tgcttatcct tgggtatctg
     6661 gtagtctttc agcttgttca gttcatccca agtcaggaag attacttttt tcgaggtggt
     6721 tttcagtttc ggtttgaacg tatcgtatgc aatgttctga tgatgtcctt tcttgaagct
     6781 ccagcgcagg aaccatttga ggaatcccat ttgcttgccg atggtgctgt ttctcatatc
     6841 cttggtgtca cgcaggaagt tgacgtattc gttcaatcca aactcgttga aatagttgaa
     6901 cgttgcatcc tccttgaact ctttgaggtg gttcctcact gctgcaaatt tttcataggt
     6961 ggatgccgtc cagttattct ggttaccgca ctcttttaca aactcatcga acacctccca
     7021 aaagctgaca ggggcttctt ccggctgttc ttcgctggtg tctttcattc tcatgttgaa
     7081 agcttccttc aactgttggg tcgttggcat gacctcctgc acctcaaatt ccttgaaaat
     7141 attctggatt tcggcatagt atttcagcaa gtccgtattg atttcggctg cactttgctt
     7201 tagcttgttg gtacatccgc tctttacccg ctgcttatct gcatcccatt tggctacgtc
     7261 aatccggtag cccgttgtaa actcgatgcg ttggctggca aagatgacac gcatacggat
     7321 gggtacgttc tctacgattg gcacaccgtt ctttttccgg ctctccaatg caaaaatgat
     7381 gttgcgcttg atattcataa ttgggtgcgt ttgaaattct acacccaaat atacacccaa
     7441 ttattgagat agcaaaagac atttagaaac atttactttt actctatatt gtaatttaca
     7501 cttgattatc agtcgtttgc agtcttatga tattctgtga aagtataagt tcgagagcct
     7561 gtctctccgc aaaaaacgct gaaaatcagc agattgcaaa acaaacaccc tgttttacac
     7621 ccaagaatgt aaagtcggct gtttttgttt tatttaagat aatacaacca ctacataata
     7681 aaagagtagc gatattaaaa gaatccgatg agaaaagact aatatttatc tatccattca
     7741 gtttgatttt tcaggacttt acatcgtcct gaaagtattt gttggtaccg gtaccgagga
     7801 cgcgtaaaca tttacagttg catgtggcct attgttttta gccgttaaat attttataac
     7861 tattaaatag cgatacaaat tgttcgaaac taatattgtt tatatcatat attctcgcat
     7921 gttttaaagc tttattaaat tgattttttg taaacagttt ttcgtactct ttgttaaccc
     7981 atttcattac aaaagtttca tatttttttc tctctttaaa tgccattttt gctggctttc
     8041 tttttaatac aattaatgtg ctatccactt taggttttgg atggaaataa tacctaggaa
     8101 tttttgctaa tatagaaata tctacctctg ccattaacag caatgctagt gatctgtttg
     8161 tatctaataa cattttagca aaaccatatt ccactattaa ataacttatt gtggctgaac
     8221 tttcaaaaac aatttttcga attatatttg tgcttatgtt gtaaggtatg ctgccaaata
     8281 ttttatatgg attgtggcta ggaaatgtaa atttcagtat atcatcattt actatttgat
     8341 agttaggata atttaagagc ttattacgag ttacctcaca taatttagaa tcaatttcta
     8401 tcgccgttac aaaattacat ctctttacca atccagcagt aaaatgacct ttccctgcac
     8461 ctatttcaaa gatgttatct ttttcatcta aacttatgca attcattatt ttttctatgt
     8521 gatattttga agtaataaaa ttttgactat cttttatatt tactttgttc attataacct
     8581 ctccttaatt tattgcatct cttttcgaat atttatgttt tttgagaaaa gaacgtactc
     8641 atggttcatc ccgatatgcg tatcggtctg tatatcagca actttctatg tgtttcaact
     8701 acaatagtca tctattctca tctttctgag tccaccccct gcaaagcccc tctttacgac
     8761 ataaaaattc ggtcggaaaa ggtatgcaaa agatgtttct ctctttaaga gaaactcttc
     8821 gggatgcaaa aatatgaaaa taactccaat tcaccaaatt atatagcgac ttttttacaa
     8881 aatgctaaaa tttgttgatt tccgtcaagc aattgttgag caaaaatgtc ttttacgata
     8941 aaatgatacc tcaatatcaa ctgtttagca aaacgatatt tctcttaaag agagaaacac
     9001 ctttttgttc accaatcccc gacttttaat cccgcggcca tgattgaaaa aggaagagta
     9061 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9121 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9181 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9241 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9301 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9361 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9421 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9481 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9541 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9601 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9661 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9721 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9781 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9841 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9901 cactgattaa gcattggtaa ctgtcagacc aagtttactc ataacgcgtc aattcgaggg
     9961 ggatcaattc cgtgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc
    10021 gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt
    10081 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
    10141 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
    10201 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
    10261 taatgacccc gaagcagggt tatgcagcgg aaaacggaat tgatccggcc acgatgcgtc
    10321 cggcgtagag gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca
    10381 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa
    10441 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt
    10501 actaagctct catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta
    10561 aggttttaag ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt
    10621 aacggttgtg gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa
    10681 agcaattttg agtgacacag gaacacttaa cggctgacat gggaattccc ctccaccgcg
    10741 gtggtacaaa gaaaattcga caaactgtta tttttctatc tatttatttg aattgtgagc
    10801 ggataacaat tacctttgtc ggcaattgtg agcggataac aattaaataa agatattct
//
