LOCUS       sequence-126163-       10858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), 
            pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 10858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM710
FEATURES             Location/Qualifiers
     source          1..10858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(104..123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            877..986
                     /note="incP origin of transfer"
     rep_origin      1157..1545
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    1619..1635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1652..1668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(1713..1739,1740..1742,1743..4229,4230..4232,
                     4233..5819)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        5979..6054
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             6499..7581
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6518..6537)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(10744..10812,10813..10858,1..746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
       61 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcataa cgcgtcaatt
      781 cgagggggat caattccgtg ataggtgggc tgcccttcct ggttggcttg gtttcatcag
      841 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
      901 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
      961 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
     1021 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
     1081 tcgctataat gaccccgaag cagggttatg cagcggaaaa cggaattgat ccggccacga
     1141 tgcgtccggc gtagaggatc tgaagatcag cagttcaacc tgttgatagt acgtactaag
     1201 ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga
     1261 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt
     1321 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag
     1381 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta
     1441 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc
     1501 tctcaaagca attttgagtg acacaggaac acttaacggc tgacatggga attcccctcc
     1561 accgcggtgg tacaaagaaa attcgacaaa ctgttatttt tctatctatt tatttgaatt
     1621 gtgagcggat aacaattacc tttgtcggca attgtgagcg gataacaatt aaataaagat
     1681 attctcgtca aacaaatata aataatataa acatggataa gaaatactca ataggcttag
     1741 ctatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag gttccgtcta
     1801 aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg
     1861 ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta
     1921 gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga
     1981 tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag
     2041 acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg
     2101 agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg
     2161 atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga
     2221 ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac
     2281 aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag
     2341 cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc
     2401 ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc
     2461 ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata
     2521 cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt
     2581 ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta agagtaaata
     2641 ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc
     2701 aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa
     2761 tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag
     2821 aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat
     2881 tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta
     2941 ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt
     3001 atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt
     3061 attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg
     3121 aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat
     3181 catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa
     3241 aacatagttt gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg
     3301 ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg
     3361 atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca
     3421 aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt
     3481 cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg
     3541 aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg
     3601 agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac
     3661 agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta
     3721 ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca
     3781 atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa
     3841 aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta
     3901 gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag
     3961 taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa
     4021 ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag
     4081 aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa
     4141 agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata
     4201 ttaatcgttt aagtgattat gatgtcgatg ccattgttcc acaaagtttc cttaaagacg
     4261 attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg
     4321 ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca
     4381 agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg
     4441 aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc
     4501 atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta
     4561 ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt
     4621 tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa
     4681 atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct
     4741 atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag
     4801 gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa
     4861 ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg
     4921 gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc
     4981 cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa
     5041 ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa
     5101 aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg
     5161 aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg
     5221 aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag
     5281 ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc
     5341 gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa
     5401 gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag
     5461 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta
     5521 ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag
     5581 ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta
     5641 ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa
     5701 caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc
     5761 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactgaa
     5821 ttaatgcggc tgcaattttt ttgggcgggg ccgcccaaaa aaatcctagc accctgcagc
     5881 agtactgctt gaccataaga acaaaaaaac ttccgataaa gtttggaaga taaagctaaa
     5941 agttcttatc tttgcagtct ggaatgaatt ggcctatggt tttagagcta gaaatagcaa
     6001 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     6061 tctagatgat gattaaggat cttgcgtaca atttgacgat cgtttcacgc taagatgctg
     6121 gctaattgcc tatcttccag tgatggaaca gcatttgtgc attggctgca acaatagcct
     6181 tgatctggaa gaagcaatga aagctgctgt taagtctccg aatcaggtat tgttcctgac
     6241 aggtgtattc ccatccggta aacgcggata ctttgcagtt gatctgactc aggaataaat
     6301 tataaattaa ggtaagaaga ttgtaggata agctaatgaa atagaaaaag gatgccgtca
     6361 cacaacttgt cggcattctt ttttgtttta ttagttgaaa atatagtgaa aaagttgcct
     6421 aaatatgtat gttaacaaat tatttgtcgt aactttgcac tccaaatctg tttttaacat
     6481 atggcactag tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     6541 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     6601 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     6661 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     6721 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     6781 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
     6841 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     6901 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     6961 cccatcaaca gtattatttt ctcccatgag gacggtacgc gactgggcgt ggagcatctg
     7021 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     7081 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     7141 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     7201 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     7261 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     7321 gataccgagg acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     7381 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     7441 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     7501 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7561 cgactggaaa gcgggcagtg agctttcctc ggtaccaaat tccagaaaag aggcctcccg
     7621 aaaggggggc cttttttcgt tttggtccta cttgtgcctg ttctatttcc gaaccgaccg
     7681 cttgtatgaa tccatcaaaa ttcgttttct ctatgttgga ttccttgttg ctcatattgt
     7741 gatgataatt tctacaaata tagtcattgg taactatcta tgaaactgtt tgatactttt
     7801 atagttgatt aaacttgttc atggcatttg ccttaatatc atccgctatg tcaatgtagg
     7861 gtttcatagc tttgtagtcg ctgtgtcccg tccatttcat gaccacctgt gccgggattc
     7921 cgagagccag cgcattgcag atgaatgtcc ttcttcctgc atgggtactg agcaaagcgt
     7981 atttgggtgt gacttcatca atacgttcat ttcccttgta gtaggtttcc cgtacaggct
     8041 cgttgatttc tgccagttcg cccagctctt tcaggtaatc gttcatcttc tggttgctga
     8101 tgacgggcag agccatgtaa ttctcgaaat ggatgtcctt gtatttgtcc agtatggctt
     8161 tgctgtattt gttcagttca atcgtcaggc tgtcggcagt cttgactgtg gttatttcga
     8221 tgtggtcgga cttcacatcg cttcttttca gattgcgaac atccgaatac cgcaaactcg
     8281 taaagcagca gaacaggaaa acatcacgca cacgttccag gtattgctta tccttgggta
     8341 tctggtagtc tttcagcttg ttcagttcat cccaagtcag gaagattact tttttcgagg
     8401 tggttttcag tttcggtttg aacgtatcgt atgcaatgtt ctgatgatgt cctttcttga
     8461 agctccagcg caggaaccat ttgaggaatc ccatttgctt gccgatggtg ctgtttctca
     8521 tatccttggt gtcacgcagg aagttgacgt attcgttcaa tccaaactcg ttgaaatagt
     8581 tgaacgttgc atcctccttg aactctttga ggtggttcct cactgctgca aatttttcat
     8641 aggtggatgc cgtccagtta ttctggttac cgcactcttt tacaaactca tcgaacacct
     8701 cccaaaagct gacaggggct tcttccggct gttcttcgct ggtgtctttc attctcatgt
     8761 tgaaagcttc cttcaactgt tgggtcgttg gcatgacctc ctgcacctca aattccttga
     8821 aaatattctg gatttcggca tagtatttca gcaagtccgt attgatttcg gctgcacttt
     8881 gctttagctt gttggtacat ccgctcttta cccgctgctt atctgcatcc catttggcta
     8941 cgtcaatccg gtagcccgtt gtaaactcga tgcgttggct ggcaaagatg acacgcatac
     9001 ggatgggtac gttctctacg attggcacac cgttcttttt ccggctctcc aatgcaaaaa
     9061 tgatgttgcg cttgatattc ataattgggt gcgtttgaaa ttctacaccc aaatatacac
     9121 ccaattattg agatagcaaa agacatttag aaacatttac ttttactcta tattgtaatt
     9181 tacacttgat tatcagtcgt ttgcagtctt atgatattct gtgaaagtat aagttcgaga
     9241 gcctgtctct ccgcaaaaaa cgctgaaaat cagcagattg caaaacaaac accctgtttt
     9301 acacccaaga atgtaaagtc ggctgttttt gttttattta agataataca accactacat
     9361 aataaaagag tagcgatatt aaaagaatcc gatgagaaaa gactaatatt tatctatcca
     9421 ttcagtttga tttttcagga ctttacatcg tcctgaaagt atttgttggt accggtaccg
     9481 aggacgcgta aacatttaca gttgcatgtg gcctattgtt tttagccgtt aaatatttta
     9541 taactattaa atagcgatac aaattgttcg aaactaatat tgtttatatc atatattctc
     9601 gcatgtttta aagctttatt aaattgattt tttgtaaaca gtttttcgta ctctttgtta
     9661 acccatttca ttacaaaagt ttcatatttt tttctctctt taaatgccat ttttgctggc
     9721 tttcttttta atacaattaa tgtgctatcc actttaggtt ttggatggaa ataataccta
     9781 ggaatttttg ctaatataga aatatctacc tctgccatta acagcaatgc tagtgatctg
     9841 tttgtatcta ataacatttt agcaaaacca tattccacta ttaaataact tattgtggct
     9901 gaactttcaa aaacaatttt tcgaattata tttgtgctta tgttgtaagg tatgctgcca
     9961 aatattttat atggattgtg gctaggaaat gtaaatttca gtatatcatc atttactatt
    10021 tgatagttag gataatttaa gagcttatta cgagttacct cacataattt agaatcaatt
    10081 tctatcgccg ttacaaaatt acatctcttt accaatccag cagtaaaatg acctttccct
    10141 gcacctattt caaagatgtt atctttttca tctaaactta tgcaattcat tattttttct
    10201 atgtgatatt ttgaagtaat aaaattttga ctatctttta tatttacttt gttcattata
    10261 acctctcctt aatttattgc atctcttttc gaatatttat gttttttgag aaaagaacgt
    10321 actcatggtt catcccgata tgcgtatcgg tctgtatatc agcaactttc tatgtgtttc
    10381 aactacaata gtcatctatt ctcatctttc tgagtccacc ccctgcaaag cccctcttta
    10441 cgacataaaa attcggtcgg aaaaggtatg caaaagatgt ttctctcttt aagagaaact
    10501 cttcgggatg caaaaatatg aaaataactc caattcacca aattatatag cgactttttt
    10561 acaaaatgct aaaatttgtt gatttccgtc aagcaattgt tgagcaaaaa tgtcttttac
    10621 gataaaatga tacctcaata tcaactgttt agcaaaacga tatttctctt aaagagagaa
    10681 acaccttttt gttcaccaat ccccgacttt taatcccgcg gccatgattg aaaaaggaag
    10741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgg
//
