LOCUS       sequence-126166-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the 5'-untranslated region of the Arabidopsis thaliana 
            actin2 gene as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: p5'UTRAct2 (GB0193)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9..18
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(779..797)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(780..797)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(815..831)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(815..831)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(828..850)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    839..855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tccggccgcc atggcggccg cgggaattcg attgggcgat gagtggtctc gtactattgt
       61 ctcgttgtcc tcctcacttt catcagccgt tttgaatctc cggcgacttg acagagaaga
      121 acaaggaaga agactaagag agaaagtaag agataatcca ggagattcat tctccgtttt
      181 gaatcttcct caatctcatc ttcttccgct ctttctttcc aaggtaatag gaactttctg
      241 gatctacttt atttgctgga tctcgatctt gttttctcaa tttccttgag atctggaatt
      301 cgtttaattt ggatctgtga acctccacta aatcttttgg ttttactaga atcgatctaa
      361 gttgaccgat cagttagctc gattatagct accagaattt ggcttgacct tgatggagag
      421 atccatgttc atgttacctg ggaaatgatt tgtatatgtg aattgaaatc tgaactgttg
      481 aagttagatt gaatctgaac actgtcaatg ttagattgaa tctgaacact gtttaaggtt
      541 agatgaagtt tgtgtataga ttcttcgaaa ctttaggatt tgtagtgtcg tacgttgaac
      601 agaaagctat ttctgattca atcagggttt atttgactgt attgaactct ttttgtgtgt
      661 ttgcagctca taaaaaaatg cgagaccact catcgcccaa tcactagtga attcgcggcc
      721 gcctgcaggt cgaccatatg ggagagctcc caacgcgttg gatgcatagc ttgagtattc
      781 tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      841 gttatccgct cacaattcca cacaacata
//
