LOCUS       sequence-126167-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM731
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cacaggaaca cttaacggct gacatgggaa ttcccctcca ccgcggtggg ttccatcact
       61 ggaagatagg caattagtag aagcaatttg attagtaatc taaaagcact ccgataacga
      121 ttagagtgct tttagattgt ttatcattaa ttatcaaagc aagtgcagtt cattatgcca
      181 ggatgcgctc gcacagacgc caacccgtga caccgttgat ggtcacgcgg aacagcaggc
      241 tgccgtccgg gttaatcaga cgctcgtcaa taatcttatt gccattccac agggtaccgg
      301 tgacggtgat ctttttaccg tcaaaaacgg caatgccttc atacggacgg ccgaaatagt
      361 caatcatatt cggggtcaca ccatcaatca ccagcgtacc gtaatgcaag ataaccttga
      421 agtggtgatc gtcaactggg tagacgactt taaagatttt ctcgatttga cccatttggt
      481 caccgctcag gccttcgtac gggatgatca catgaatatc aattttcaga ccgttctcgc
      541 cggacagaac gatgcgctga atcggcgtca cgctcacacc caggttctgg aacagagagc
      601 ttacgccacc ctgttccagg acttgatcca aattataacc cgcggtctga cgccaatcgc
      661 caacaaaatc ttccagagta a
//
