LOCUS       sequence-126168-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM732
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac cgcggtgggt
       61 tccatcactg gaagataggc aattagtaga agcaatttga ttagtaatct aaaagcactc
      121 cgataacgat tagagtgctt ttagattgtt tatcattaat tatcaaagca agtgcagttc
      181 attatgccag gatgcgctcg cacagacgcc aacccgtgac accgttgatg gtcacgcgga
      241 acagcaggct gccgtccggg ttaatcagac gctcgtcaat aatcttattg ccattccaca
      301 gggtaccggt gacggtgatc tttttaccgt caaaaacggc aatgccttca tacggacggc
      361 cgaaatagtc aatcatattc ggggtcacac catcaatcac cagcgtaccg taatgcaaga
      421 taaccttgaa gtggtgatcg tcaactgggt agacgacttt aaagattttc tcgatttgac
      481 ccatttggtc accgctcagg ccttcgtacg ggatgatcac atgaatatca attttcagac
      541 cgttctcgcc ggacagaacg atgcgctgaa tcggcgtcac gctcacaccc aggttctgga
      601 acagagagct tacgccaccc tgttccagga cttgatccaa attataaccc gcggtctgac
      661 gccaatcgcc aacaaaatct tccagagtaa
//
