LOCUS       sequence-126170-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive 
            NanoLuc, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM733
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgagtgacac aggaacactt aacggctgac atgggaattc ccctccaccg cggtgggttc
       61 catcactgga agataggcaa ttagtagaag caatttgatt agtaatctaa aagcactccg
      121 ataacgatta gagtgctttt agattgttta tcattaatta tcaaagcaag tgcagttcat
      181 tatgccagga tgcgctcgca cagacgccaa cccgtgacac cgttgatggt cacgcggaac
      241 agcaggctgc cgtccgggtt aatcagacgc tcgtcaataa tcttattgcc attccacagg
      301 gtaccggtga cggtgatctt tttaccgtca aaaacggcaa tgccttcata cggacggccg
      361 aaatagtcaa tcatattcgg ggtcacacca tcaatcacca gcgtaccgta atgcaagata
      421 accttgaagt ggtgatcgtc aactgggtag acgactttaa agattttctc gatttgaccc
      481 atttggtcac cgctcaggcc ttcgtacggg atgatcacat gaatatcaat tttcagaccg
      541 ttctcgccgg acagaacgat gcgctgaatc ggcgtcacgc tcacacccag gttctggaac
      601 agagagctta cgccaccctg ttccaggact tgatccaaat tataacccgc ggtctgacgc
      661 caatcgccaa caaaatcttc cagagtaa
//
