LOCUS       sequence-12618-e       10471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10471)
  TITLE     Gerardo Morfini plasmids
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FSynIGW
FEATURES             Location/Qualifiers
     source          1..10471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3758..3814
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    join(3816..4390,4391..4393,4394..4402)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3983..4000)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4210..4229
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4403..4405,4406..4408,4409..5122)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4403..4405,4406..4408,4409..5119)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4448..4469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4709..4728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5056..5077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5163..5751
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5216..5236)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5634..5645)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5754..5770)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5755..5771)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6276..6456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6482..6499)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6488..6712
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6758..7186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6845..6864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7055..7076
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7195..7215)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7200..7529
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7380..7515
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7442..7461
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7577..7624
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7643..8017
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8147..8268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8184..8203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8238..8257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8317..8333)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8317..8333)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8330..8352)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8341..8357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8365..8371,8372..8389,8390..8395))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8410..8431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8548..8565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8719..9307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8799..8818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9478..10269,10270..10338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10101..10120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10339..10443)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggttttgg ctacgtccag agcagaggaa tgagggcatg
     2641 tagactaaat atgttcgtgt ggaagaggct gaatacacat cagagttagt gctgcaggaa
     2701 atgcttctgc attgcatacc cagagtttcc ttgctcatct gagagcatgt gttttttcca
     2761 gatgtgtgta cttgtgtgag attctctggg tgtgtgtcaa tgtgttgcct gaacgtgcat
     2821 tgctcaatat gctcatgtgt gttaccctgg gcttgtacat ctacatatat acctggatgc
     2881 ccgtgtgttc tgtgatgtac atataccctg tgtcattcct tgtttttcta tttgtgttat
     2941 tccatgtgtt ccttcaggct ctcactaccc aagtgtccac ctccgcctgt ctggtgatgt
     3001 ttacgctacc ccgtgctctt ttctttgcct gacagtgttg tcgtggaaga catctcgcca
     3061 ggaacactgc agtaaggaga atttctagtt ttatgttccc ctccgagtat gcttctatcc
     3121 cgaccctcaa ccccaaaatg ccttcagagg tgaaaatcaa cactggaaac acaagtatct
     3181 gggaagggta acaatgcaag ttagcctgag gatttaggag gaggctgaaa aacagagtag
     3241 gagccttact acgggtccag accctacgga caagaacccc cactcccact ccccaaattg
     3301 cgcattccct cccccatcag agggggaggg gaagaggatg cagcgcggcg cggcgcgtgc
     3361 gcactgtcgg atttagtacc gcggacagag ccttcgcccc cgctgccggc gcgcgccacc
     3421 acctccccag caccaaaggc gggctgacgt cactctccag ccctccccaa actcccctac
     3481 ctcaccgcct tggtcgcgtc cgtgcagcgg tgagtccagt cgggccgcac cacaagaggt
     3541 gcaagatagg ggggtgcagg cgcgaccata cgctctgcgg cggcagagcc tcagcgctgc
     3601 ctcagtctgc agcgggcagc agaggagtcg cgtcgtgcca gagagcgccg ccgtgctcct
     3661 gagccccttg cgctccgccc ccgcggccca ccgacccact gccccttgga tccccgggta
     3721 ccggtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa
     3781 ttctgcagtc gacggtaccg cgggcccggg atccgcccct ctccctcccc cccccctaac
     3841 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3901 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3961 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     4021 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     4081 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     4141 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4201 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4261 ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg
     4321 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4381 cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca ccggggtggt
     4441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4501 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4561 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     4621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4801 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     4861 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     4921 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4981 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5101 catggacgag ctgtacaagt aaagcggccg cgactctaga attcgatatc aagcttatcg
     5161 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5221 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5281 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5341 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5401 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5461 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5521 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     5581 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5641 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5701 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     5761 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     5821 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     5881 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5941 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6001 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6061 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6121 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6181 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     6241 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     6301 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6361 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6421 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     6481 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6541 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6601 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6661 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6721 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6781 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6841 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6901 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6961 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7021 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7081 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7141 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7201 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7261 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7321 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7381 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7441 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7501 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7561 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7621 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     7681 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     7741 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     7801 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     7861 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     7921 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     7981 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8041 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8101 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8161 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8221 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8281 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8341 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8401 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8461 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8521 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8881 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9121 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10441 gcgcacattt ccccgaaaag tgccacctga c
//
