LOCUS       sequence-126183-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-STRHISNDHFR
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(480..500)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(528..545)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(555..578)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             595..617
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(672..691)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(673..691)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcacgacgct cgaggatttc gaaacaatag ctatgcgagt tctgcgcatc agcatcgtgg
       61 aattcgctga ataccgattc ccagtcatcc ggctcgtaat ccggaaaatg ggtgtcgcct
      121 tccacttctg catcgatatg cgtcagatac agtttttgcg cttttggcaa gaactgttca
      181 taaacgcgtc cgccgccaat caccatgatt tctggtacgt caccacacgc cgcgatggct
      241 tcgtcgaccg atttaaccca ggtaacccga tcatcggtcc cgggttgact gctgaggata
      301 atatttttgc ggccgggcaa aggcctaccg attgattccc aggtatggcg ccccataatc
      361 acgggtttat ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccatggc
      421 atggcgtttt ccatgccgat aacgcgatct accgctagcg ccgcaatcag actgatggat
      481 ccctggaagt acaggttttc accaccacca ccaccagatc cgctgctgtg atgatgatga
      541 tgatggctag atcctttctc gaactgcggg tggctccagc ttgccatatg tatatctcct
      601 tcttaaagtt aaacaaaatt attgaattcc aattggagct ctccggagcc ggagggaaac
      661 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgatgca tggtgccaac
      721 acctacatct gtattaacga agcgctggca
//
