LOCUS       sequence-126205-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4YWQ
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg catgcctgta caagctccac
      121 aatggacgga tttcctctcc tgcccaattt gcactcagac tttcgacgaa acaattcgaa
      181 agcccatcag tttgggttgt ggccatactg tctgcaagat gtgcctgaat aaactccacc
      241 gcaaggcttg cccatttgac cagaccacta tcaatacaga cattgagctc ctccctgtga
      301 actcagcatt gctgcagctc gtgggtgctc aggtccctga gcagcagcct attactttgt
      361 gtagtggggt tgaagacaca aagcattatg aggaagccaa gaaatgtgta gaagaattag
      421 cattgtactt aaaaccgctc agcagtgcta gaggagtggg tctgaacagc actactcaga
      481 gtgttctgag tcgcccaatg cagaggaagc ttgtgactct agtccactgt caactagtag
      541 aagaagaagg caggattcgt gccatgaggg cagctcgatc tttaggtgaa cgaacagtta
      601 cagagctcat tctccagcac cagaatcctc agcaactctc ttccaatctt tgggcagcag
      661 taagggctag gggatgccag ttccttggac cagcaatgca ggaggaagct ttaaagttgg
      721 ttctgctggc cttagaagat ggttctgctt tgtcaagaaa agtattggtt ctgtttgtgg
      781 tgcaaagatt ggagccacgg tttcctcaag cctctaaaac tagcattggg catgttgttc
      841 agctccttta tagagcctcc tgtttcaagg tcaccaaacg tgatgaagac tcttctttga
      901 tgcagctgaa agaagaattt agaacctatg aagctctgcg gcgagaacat gactcccaga
      961 tagtgcagat tgctatggaa gcaggcttac gaattgcacc ggaccagtgg tcctctttgc
     1021 tttatggaga ccagtctcac aaatctcata tgcagtccat t
//
