LOCUS       sequence-126211-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM585
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    530..546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    553..569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgatggtca cgcggaacag caggctgccg tccgggttaa tcagacgctc gtcaataatc
       61 ttattgccat tccacagggt accggtgacg gtgatctttt taccgtcaaa aacggcaatg
      121 ccttcatacg gacggccgaa atagtcaatc atattcgggg tcacaccatc aatcaccagc
      181 gtaccgtaat gcaagataac cttgaagtgg tgatcgtcaa ctgggtagac gactttaaag
      241 attttctcga tttgacccat ttggtcaccg ctcaggcctt cgtacgggat gatcacatga
      301 atatcaattt tcagaccgtt ctcgccggac agaacgatgc gctgaatcgg cgtcacgctc
      361 acacccaggt tctggaacag agagcttacg ccaccctgtt ccaggacttg atccaaatta
      421 taacccgcgg tctgacgcca atcgccaaca aaatcttcca gagtaaaaac catgtttata
      481 ttatttatat ttgtttgacg agaatatctt tatttgccga caaaggtaat tgttatccgc
      541 tcacaattca aattgttatc cgctcacaat ttaaatagat agaaaaataa cagtttgtcg
      601 aattttcttt gtaccaccgc ggtggagggg aattcccatg tcagccgtta agtgttcctg
      661 tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct
      721 tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct
      781 tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc
      841 aaacatga
//
