LOCUS       sequence-126212-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM596
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    525..541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    581..597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gacaccgttg atggtcacgc ggaacagcag gctgccgtcc gggttaatca gacgctcgtc
       61 aataatctta ttgccattcc acagggtacc ggtgacggtg atctttttac cgtcaaaaac
      121 ggcaatgcct tcatacggac ggccgaaata gtcaatcata ttcggggtca caccatcaat
      181 caccagcgta ccgtaatgca agataacctt gaagtggtga tcgtcaactg ggtagacgac
      241 tttaaagatt ttctcgattt gacccatttg gtcaccgctc aggccttcgt acgggatgat
      301 cacatgaata tcaattttca gaccgttctc gccggacaga atgatgcgct gaatcggcgt
      361 cacgctcaca cccaggttct ggaacagaga gcttacgcca ccctgttcca ggacttgatc
      421 caaattataa cccgcggtct gacgccaatc gccaacaaaa tcttccagag taaaaaccat
      481 gtttatatta tttatatttg tttgacgaga atatctttat ttaattgtta tccgctcaca
      541 attgccgaca aaggtacata actaaagttt cccacccaaa ttgttatccg ctcacaattt
      601 aaatagatag aaaaataaca gtttgtcgaa ttttctttgt accaccgcgg tggaggggaa
      661 ttcccatgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag
      721 ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt
      781 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
      841 ttgctgaatt tatattaatt ttattg
//
