LOCUS       sequence-126214-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  TITLE     SGC Toronto Q1 Q2 2015
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 4Z0O
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             305..322
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(375..393)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      389..436
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      473..928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(560..579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     770..791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg catcatcatc
       61 atcatcacag cagcggcaga gaaaacttgt atttccaggg cgtggaaaac atgtatgtaa
      121 acaaagtgtg ggttcaatgt gagaatgaaa attgtttgaa atggagattg ttatcaagtg
      181 aggattcagc caaggttgat catgatgaac catggtactg cttcatgaac actgattcaa
      241 gatataataa ctgctcaatt tctgaagaag acttctgatg acgaagcttg cggccgcact
      301 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      361 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      421 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
      721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
      781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
      841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
      901 tttaacaaaa tattaacgct tacaatttta ggtggcactt ttcggggaaa tgtgcgcgga
      961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     1021 ttagaaaaac tcatcgagca
//
